Bcl-6 Antibody (H-12): sc-365618
- Bcl-6 Antibody (H-12) is a mouse monoclonal IgG1 κ, cited in 6 publications, provided at 200 µg/ml
- specific for an epitope mapping between amino acids 679-706 at the C-terminus of Bcl-6 of human origin
- Anti-Bcl-6 Antibody (H-12) is recommended for detection of Bcl-6 of mouse, rat and human origin by WB, IP, IF and ELISA; also reactive with additional species, including and equine, canine, bovine and porcine
- TransCruz reagent for ChIP application (sc-365618 X, 200 µg/0.1 ml)
- Anti-Bcl-6 Antibody (H-12) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of Bcl-6 (H-12): sc-365618.
- m-IgG Fc BP-HRP and m-IgG1 BP-HRP are the preferred secondary detection reagents for Bcl-6 Antibody (H-12) for WB applications. These reagents are now offered in bundles with Bcl-6 Antibody (H-12) (see ordering information below).
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SEE ALSO...
Bcl-6 Antibody (H-12) is a mouse monoclonal IgG1 kappa light chain antibody designed to detect Bcl-6 protein, also known as BCL6 transcription repressor or B-cell lymphoma 6 protein, across mouse, rat, human, equine, canine, bovine, and porcine species, utilizing applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). Bcl-6 monoclonal antibody (H-12) specifically targets an epitope mapping to amino acids 679-706 at the C-terminus of human Bcl-6 protein, ensuring precise and reliable binding. Bcl-6 (H-12) antibody recognizes a pivotal transcriptional repressor involved in regulating gene expression by binding to Stat recognition-like DNA elements, playing an essential role in germinal center development, B cell differentiation, and T helper cell differentiation into Th1 and Th2 subsets, which are critical for adaptive immune response. Structurally, Bcl-6 contains a BTB/POZ domain responsible for protein-protein interactions and multiple zinc finger motifs that facilitate specific DNA binding, enabling interactions with various co-repressors such as SMRT and BCOR, which are crucial for transcriptional repression. These interactions are vital for modulating processes like NFκB signaling, where Bcl-6 negatively regulates NFκB expression, thereby inhibiting NFκB-mediated cellular functions important for maintaining cellular homeostasis and preventing excessive inflammatory responses. Post-translational modifications of Bcl-6, including acetylation, phosphorylation, and ubiquitination, are critical for regulating stability, localization, and transcriptional activity, with HDAC- and SIR2-dependent acetylation specifically downregulating Bcl-6 activity by inhibiting histone deacetylase complex recruitment. Dysregulation of Bcl-6 through aberrant post-translational modifications is frequently observed in non-Hodgkin′s B cell lymphomas, highlighting significance in oncogenesis and making Bcl-6 a valuable target for therapeutic intervention. Anti-Bcl-6 antibody (H-12) is available in both non-conjugated form and multiple conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and various Alexa Fluor® conjugates.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Bcl-6 Antibody (H-12) | sc-365618 | 200 µg/ml | $316.00 | |||
Bcl-6 Antibody (H-12): m-IgG Fc BP-HRP Bundle | sc-527387 | 200 µg Ab; 10 µg BP | $354.00 | |||
Bcl-6 Antibody (H-12): m-IgG1 BP-HRP Bundle | sc-532760 | 200 µg Ab; 20 µg BP | $354.00 | |||
Bcl-6 Antibody (H-12) X | sc-365618 X | 200 µg/0.1 ml | $316.00 | |||
Bcl-6 Antibody (H-12) AC | sc-365618 AC | 500 µg/ml, 25% agarose | $416.00 | |||
Bcl-6 Antibody (H-12) HRP | sc-365618 HRP | 200 µg/ml | $316.00 | |||
Bcl-6 Antibody (H-12) FITC | sc-365618 FITC | 200 µg/ml | $330.00 | |||
Bcl-6 Antibody (H-12) PE | sc-365618 PE | 200 µg/ml | $343.00 | |||
Bcl-6 Antibody (H-12) Alexa Fluor® 488 | sc-365618 AF488 | 200 µg/ml | $357.00 | |||
Bcl-6 Antibody (H-12) Alexa Fluor® 546 | sc-365618 AF546 | 200 µg/ml | $357.00 | |||
Bcl-6 Antibody (H-12) Alexa Fluor® 594 | sc-365618 AF594 | 200 µg/ml | $357.00 | |||
Bcl-6 Antibody (H-12) Alexa Fluor® 647 | sc-365618 AF647 | 200 µg/ml | $357.00 | |||
Bcl-6 Antibody (H-12) Alexa Fluor® 680 | sc-365618 AF680 | 200 µg/ml | $357.00 | |||
Bcl-6 Antibody (H-12) Alexa Fluor® 790 | sc-365618 AF790 | 200 µg/ml | $357.00 | |||
Bcl-6 (H-12) Neutralizing Peptide | sc-365618 P | 100 µg/0.5 ml | $68.00 |