Bcl-6 Antibody (7D1): sc-130916

Bcl-6 Antibody (7D1) is a rat monoclonal IgG2a antibody engineered to detect Bcl-6 protein, also known as BCL6 transcription repressor or B-cell lymphoma 6 protein, across mouse, rat, and human species using western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and flow cytometry (FCM) techniques. Bcl-6 monoclonal antibody (7D1) targets an epitope corresponding to amino acids 261-386 of the mouse Bcl-6 protein, ensuring precise and reliable binding. Bcl-6 is a critical transcriptional repressor involved in regulating gene expression by binding to Stat recognition-like DNA elements, which plays a pivotal role in germinal center development and the differentiation of T helper cells into Th1 and Th2 subsets, essential processes in the adaptive immune response. Post-translational modifications of Bcl-6, such as acetylation, phosphorylation, and ubiquitination, are crucial for regulating stability, localization, and transcriptional activity. HDAC- and SIR2-dependent acetylation of Bcl-6 modulates activity by inhibiting the recruitment of histone deacetylase complexes, thereby downregulating repressive functions. These modifications are vital for the fine-tuning of Bcl-6′s role in various cellular processes, including the negative regulation of NFκB expression, which is important for controlling inflammatory responses and maintaining immune system balance. Dysregulation of Bcl-6 through aberrant post-translational modifications is frequently observed in non-Hodgkin′s B cell lymphomas, highlighting significance in oncogenesis and making Bcl-6 a valuable target for therapeutic intervention. Anti-Bcl-6 antibody (7D1) is available in a non-conjugated form, providing researchers with a reliable tool to explore the multifaceted roles of Bcl-6 in immune regulation and disease, thereby facilitating advancements in both basic research and clinical applications.

The preferred Bcl-6 antibody is Bcl-6 Antibody (D-8): sc-7388.