p-CREB1 Antibody (10E9): sc-81486

p-CREB-1 Antibody (10E9) is a mouse monoclonal IgG1 kappa light chain antibody that detects p-CREB-1 in mouse, rat, human, and canine samples through western blotting (WB) and immunoprecipitation (IP) applications. anti-p-CREB-1 antibody (10E9) is available in a non-conjugated form, specifically designed to target Ser 133 phosphorylated CREB-1 and Ser 63 phosphorylated ATF-1. CREB-1, a member of the ATF/CREB transcription factor family, plays a crucial role in regulating eukaryotic gene transcription by binding to the cAMP response element (CRE) in promoter regions of target genes. This family, which includes CREB-2 (ATF-4), ATF-1, ATF-2, and ATF-3, features a highly divergent N-terminal domain and a conserved C-terminal leucine zipper that facilitates dimerization and DNA binding. Phosphorylation of CREB-1 at Ser133 by protein kinase A enhances interaction with CREB-binding protein (CBP), promoting assembly of the basal transcription complex. Such activation is particularly important in processes like gluconeogenesis, where CREB-1 interacts with nuclear coactivator PGC-1, highlighting implications in metabolic disorders like type II diabetes and dilated cardiomyopathy. CREB-1 is encoded on human chromosome 2q32.3-q34, underscoring significance in both normal physiology and disease states.

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p-CREB1 Antibody (10E9) References:

1. CREB regulates hepatic gluconeogenesis through the coactivator PGC-1.  |  Herzig, S., et al. 2001. Nature. 413: 179-83. PMID: 11557984
2. Assignment of the human gene for CREB1 to chromosome 2q32.3-q34.  |  Taylor, AK., et al. 1990. Genomics. 7: 416-21. PMID: 2142119
3. Transcription factor ATF cDNA clones: an extensive family of leucine zipper proteins able to selectively form DNA-binding heterodimers.  |  Hai, TW., et al. 1989. Genes Dev. 3: 2083-90. PMID: 2516827
4. Interaction of a common cellular transcription factor, ATF, with regulatory elements in both E1a- and cyclic AMP-inducible promoters.  |  Lin, YS. and Green, MR. 1988. Proc Natl Acad Sci U S A. 85: 3396-400. PMID: 2835770
5. Identification of a cyclic-AMP-responsive element within the rat somatostatin gene.  |  Montminy, MR., et al. 1986. Proc Natl Acad Sci U S A. 83: 6682-6. PMID: 2875459
6. Phosphorylation-induced binding and transcriptional efficacy of nuclear factor CREB.  |  Yamamoto, KK., et al. 1988. Nature. 334: 494-8. PMID: 2900470
7. p-CREB-1 at Ser 133 is a potential marker for breast cancer.  |  Xin, ZC., et al. 2020. Eur Rev Med Pharmacol Sci. 24: 11628-11638. PMID: 33275230
8. Nuclear protein CBP is a coactivator for the transcription factor CREB.  |  Kwok, RP., et al. 1994. Nature. 370: 223-6. PMID: 7913207
9. Activation of cAMP and mitogen responsive genes relies on a common nuclear factor.  |  Arias, J., et al. 1994. Nature. 370: 226-9. PMID: 8028671
10. Dilated cardiomyopathy in transgenic mice expressing a dominant-negative CREB transcription factor in the heart.  |  Fentzke, RC., et al. 1998. J Clin Invest. 101: 2415-26. PMID: 9616213

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-p-CREB1 Antibody (10E9) has 82 citations in a variety of scientific publications.

Primary antibodies like Anti-p-CREB1 Antibody (10E9) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

p-CREB1 Antibody (10E9) Specifications

Alternate Product Name: CREB

Product Clone Name: 10E9

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: Ser 133

Epitope Target: CREB-1

Epitope Species: human

Product Reactivity/Detection: Ser 133 phosphorylated CREB-1 and Ser 63 phosphorylated ATF-1

Species Reactivity/Detection: mouse, rat, human, canine

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP

Vialing Information: Each vial contains 50 µg IgG₁ kappa light chain in 0.5 ml of PBS with < 0.1% sodium azide, 0.1% gelatin, PEG and sucrose

Research Citations: 82 citations in various scientific publications

Available Conjugates/Forms for p-CREB1 Antibody (10E9): N/A

The preferred CREB1 antibody is CREB1 Antibody (D-12): sc-377154.