E2F1 Antibody (KH129): sc-56661

E2F-1 Antibody (KH129) is a mouse monoclonal IgG1 antibody engineered to recognize E2F-1 protein, also known as RBAP-1 or RBBP-3, across human, mouse, and rat species. Anti-E2F-1 antibody (KH129) is raised against a recombinant protein epitope encompassing amino acids 386-409 of human E2F-1, ensuring effective detection in applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and immunohistochemistry with paraffin-embedded sections (IHCP). E2F-1 is a pivotal transcription factor localized primarily in the nucleus, where E2F-1 orchestrates the expression of genes necessary for the transition from the G1 phase to the S phase of the cell cycle. By regulating these critical phases, E2F-1 plays an essential role in controlling cell proliferation and ensuring proper cell division. The activity of E2F-1 is modulated through interaction with the retinoblastoma protein (Rb), which binds to E2F-1 to inhibit the ability to activate gene transcription until the cell receives appropriate signals to proceed with DNA replication. Disruptions in the regulation of E2F-1, whether through genetic mutations or interactions with viral proteins, can lead to uncontrolled cell growth and are frequently associated with the development of various cancers. Understanding the nuclear localization and function of E2F-1 is crucial for elucidating the mechanisms of cell cycle control and the molecular basis of tumorigenesis. E2F-1 monoclonal antibody (KH129) provides researchers with a tool to investigate the role of E2F-1 in cell cycle dynamics, cancer progression, and the broader E2F/Rb signaling network, facilitating advancements in both basic and applied biomedical research.

The preferred E2F1 antibody is E2F1 Antibody (KH95): sc-251.