PKAα cat Antibody (A-2): sc-28315
- PKAα cat Antibody (A-2) is a mouse monoclonal IgG2a κ PKAα cat antibody, cited in 45 publications, provided at 200 µg/ml
- specific for an epitope mapping between amino acids 322-351 at the C-terminus of PKAα cat of human origin
- PKA alpha cat Antibody (A-2) is recommended for detection of PKAα catalytic subunit of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA; also reactive with additional species, including and equine, bovine and porcine; may cross-react with β and γ subunits
- Anti-PKA alpha cat Antibody (A-2) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of PKAα cat (A-2): sc-28315.
- m-IgG Fc BP-HRP, m-IgG2a BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for PKAα cat Antibody (A-2) for WB and IHC(P) applications. These reagents are now offered in bundles with PKAα cat Antibody (A-2) (see ordering information below).
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PKAα cat Antibody (A-2) is a mouse monoclonal IgG2a kappa light chain antibody engineered to target the protein kinase A (PKA) alpha catalytic subunit (PRKACA) with exceptional precision. PKAα cat monoclonal antibody (A-2) recognizes an epitope mapped to amino acids 322-351 at the C-terminus of the human PKAα catalytic subunit, ensuring robust detection in western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry with paraffin-embedded sections (IHCP), and enzyme-linked immunosorbent assay (ELISA) applications across multiple species, including mouse, rat, and human, as well as additional species such as equine, bovine, and porcine, though may exhibit cross-reactivity with the β and γ subunits of PKA. PKA plays a pivotal role in cellular signaling by mediating responses to the second messenger cyclic AMP (cAMP), which is essential for regulating a wide array of physiological processes including cell proliferation, ion transport, metabolism, and gene transcription. Upon binding of cAMP, the regulatory subunits of the tetrameric PKA holoenzyme dissociate, releasing the active catalytic subunits that phosphorylate target proteins such as the transcription factor CREB on serine 133. This phosphorylation event is critical for the initiation of gene transcription, linking extracellular signals to the regulation of gene expression and ultimately influencing cellular function and behavior. Anti-PKAα cat antibody (A-2) is available in both non-conjugated form and multiple conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and various Alexa Fluor® conjugates, providing versatile options to accommodate diverse experimental requirements and facilitating advanced studies into the mechanisms of PKA-mediated cellular processes.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
PKAα cat Antibody (A-2) References:
- Cyclic AMP stimulates somatostatin gene transcription by phosphorylation of CREB at serine 133. | Gonzalez, GA. and Montminy, MR. 1989. Cell. 59: 675-80. PMID: 2573431
- A cDNA clone encoding human cAMP-dependent protein kinase catalytic subunit C alpha. | Maldonado, F. and Hanks, SK. 1988. Nucleic Acids Res. 16: 8189-90. PMID: 2843813
- Activation of protein kinase by physiological concentrations of cyclic AMP. | Beavo, JA., et al. 1974. Proc Natl Acad Sci U S A. 71: 3580-3. PMID: 4372627
- Signal transduction through the cAMP-dependent protein kinase. | Meinkoth, JL., et al. 1993. Mol Cell Biochem. 127-128: 179-86. PMID: 7935349
- Transcription factors. CREB takes CBP to tango. | Nordheim, A. 1994. Nature. 370: 177-8. PMID: 8028657
- Phosphorylation-dephosphorylation of enzymes. | Krebs, EG. and Beavo, JA. 1979. Annu Rev Biochem. 48: 923-59. PMID: 38740
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
PKAα cat Antibody (A-2) | sc-28315 | 200 µg/ml | $316.00 | |||
PKAα cat Antibody (A-2): m-IgG Fc BP-HRP Bundle | sc-528419 | 200 µg Ab; 10 µg BP | $354.00 | |||
PKAα cat Antibody (A-2): m-IgGκ BP-HRP Bundle | sc-520793 | 200 µg Ab, 40 µg BP | $354.00 | |||
PKAα cat Antibody (A-2): m-IgG2a BP-HRP Bundle | sc-547092 | 200 µg Ab; 10 µg BP | $354.00 | |||
PKAα cat Antibody (A-2) AC | sc-28315 AC | 500 µg/ml, 25% agarose | $416.00 | |||
PKAα cat Antibody (A-2) HRP | sc-28315 HRP | 200 µg/ml | $316.00 | |||
PKAα cat Antibody (A-2) FITC | sc-28315 FITC | 200 µg/ml | $330.00 | |||
PKAα cat Antibody (A-2) PE | sc-28315 PE | 200 µg/ml | $343.00 | |||
PKAα cat Antibody (A-2) Alexa Fluor® 488 | sc-28315 AF488 | 200 µg/ml | $357.00 | |||
PKAα cat Antibody (A-2) Alexa Fluor® 546 | sc-28315 AF546 | 200 µg/ml | $357.00 | |||
PKAα cat Antibody (A-2) Alexa Fluor® 594 | sc-28315 AF594 | 200 µg/ml | $357.00 | |||
PKAα cat Antibody (A-2) Alexa Fluor® 647 | sc-28315 AF647 | 200 µg/ml | $357.00 | |||
PKAα cat Antibody (A-2) Alexa Fluor® 680 | sc-28315 AF680 | 200 µg/ml | $357.00 | |||
PKAα cat Antibody (A-2) Alexa Fluor® 790 | sc-28315 AF790 | 200 µg/ml | $357.00 | |||
PKAα cat (A-2) Neutralizing Peptide | sc-28315 P | 100 µg/0.5 ml | $68.00 |