PKAα cat Antibody (C-7): sc-48412
- PKAα cat Antibody (C-7) is a mouse monoclonal IgG2a κ, cited in 5 publications, provided at 200 µg/ml
- specific for an epitope mapping between amino acids 322-351 at the C-terminus of PKAα cat of human origin
- recommended for detection of PKAα catalytic subunit of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA; also reactive with additional species, including and equine, bovine and porcine; may cross-react with cross-reactive with β and γ subunits
- See PKAα cat (A-2): sc-28315 for PKAα cat antibody conjugates, including AC, HRP, FITC, PE, Alexa Fluor® 488, 594, 647, 680 and 790.
- m-IgGκ BP-HRP is the preferred secondary detection reagent for PKAα cat Antibody (C-7) for WB and IHC(P) applications. This reagent is now offered in a bundle with PKAα cat Antibody (C-7) (see ordering information below). For additional m-IgGκ BP conjugates see our complete list of Mouse IgG Binding Proteins.
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PKAα cat Antibody (C-7) is a mouse monoclonal IgG2a kappa light chain antibody, engineered to target the protein kinase A catalytic subunit alpha (PKAα cat) with exceptional precision. Anti-PKAα cat antibody (C-7) recognizes a unique epitope mapped to amino acids 322-351 at the C-terminus of the human PKAα catalytic subunit, ensuring selective detection across multiple species including mouse, rat, human, equine, bovine, and porcine, and may also cross-react with the β and γ catalytic subunits. PKAα cat is a critical component of the cAMP-dependent protein kinase A (PKA) holoenzyme, which plays a pivotal role in mediating diverse cellular responses such as proliferation, ion transport, metabolism regulation, and gene transcription. Structurally, PKAα cat consists of a conserved kinase domain essential for catalytic activity, and a regulatory domain that modulates function through binding with cAMP. Post-translational modifications, particularly phosphorylation, are vital for regulating PKAα cat′s activity, stability, and interactions with other proteins, thereby fine-tuning the signaling pathways governed. PKAα cat monoclonal antibody (C-7) recognizes these modified forms of the protein, enhancing utility in studying the dynamic regulation of PKA in various physiological and pathological contexts. Whether used in western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry, or enzyme-linked immunosorbent assay (ELISA), PKAα cat (C-7) antibody provides researchers with a reliable tool to investigate the intricate mechanisms of PKA activation, including the release of catalytic subunits upon cAMP binding to regulatory subunits, and the subsequent phosphorylation of key substrates like the cAMP response element-binding protein (CREB) on Serine 133. By facilitating detailed analysis of PKAα cat′s structure and modifications, anti-PKAα cat antibody (C-7) supports the elucidation of signal transduction pathways and impact on cellular functions, making this reagent indispensable for advanced biomedical research.
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
PKAα cat Antibody (C-7) | sc-48412 | 200 µg/ml | $316.00 | |||
PKAα cat Antibody (C-7): m-IgGκ BP-HRP Bundle | sc-520938 | 200 µg Ab, 40 µg BP | $354.00 | |||
PKAα cat (C-7) Neutralizing Peptide | sc-48412 P | 100 µg/0.5 ml | $68.00 |