IL-6 Antibody (B-F6): sc-65327

IL-6 Antibody (B-F6) is a mouse monoclonal IgG1 antibody that detects interleukin-6 in human samples through western blotting (WB) and immunoprecipitation (IP) applications. IL-6 is a multifunctional protein composed of 212 amino acids, playing a pivotal role in host defense, immune response, and hematopoiesis. IL-6 is constitutively expressed by epidermal Langerhans cells, with expression further induced in stimulated keratinocytes, highlighting importance in the skin′s immune response. Notably, IL-6 acts as an endogenous pyrogen alongside IL-1β and tumor necrosis factor-alpha (TNFα), regulating fever response during bacterial infections. The IL-6 receptor is a trimeric complex that includes an IL-6-specific alpha chain and a homodimer of the gp130 glycoprotein, which is common to several other receptors, including those for IL-11, ciliary neurotrophic factor (CNTF), oncostatin M (OSM), and leukemia inhibitory factor (LIF). Upon IL-6 binding, the receptor complex undergoes homodimerization of gp130, leading to activation of Janus kinases JAK1 and JAK2. These kinases subsequently phosphorylate signal transducer and activator of transcription 3 (Stat3), facilitating nuclear translocation and transcription of Stat3-responsive genes, which are crucial for various cellular responses. Additionally, IL-6 has been implicated in activating the Ras/MAP kinase pathway, further influencing transcription of NFIL6, thereby underscoring significance in both inflammatory responses and cellular signaling pathways.

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IL-6 Antibody (B-F6) References:

1. Role of interleukin 6 in the growth of myeloma-derived cell lines.  |  Barut, BA., et al. 1992. Leuk Res. 16: 951-9. PMID: 1405708
2. Expression of interleukin-6 and its effects on growth of HP75 human pituitary tumor cells.  |  Borg, SA., et al. 2003. J Clin Endocrinol Metab. 88: 4938-44. PMID: 14557477
3. Interleukin-6: a multifunctional targetable cytokine in human prostate cancer.  |  Culig, Z. and Puhr, M. 2012. Mol Cell Endocrinol. 360: 52-8. PMID: 21664423
4. Interleukin-6 signaling pathway in targeted therapy for cancer.  |  Guo, Y., et al. 2012. Cancer Treat Rev. 38: 904-10. PMID: 22651903
5. Interleukin-6 gene (IL-6): a possible role in brain morphology in the healthy adult brain.  |  Baune, BT., et al. 2012. J Neuroinflammation. 9: 125. PMID: 22695063
6. Induction of IL-6 by Cytotoxic Chemotherapy Is Associated With Loss of Lean Body and Fat Mass in Tumor-free Female Mice.  |  Elsea, CR., et al. 2015. Biol Res Nurs. 17: 549-57. PMID: 25406461
7. Exercise-induced increase in IL-6 level enhances GLUT4 expression and insulin sensitivity in mouse skeletal muscle.  |  Ikeda, SI., et al. 2016. Biochem Biophys Res Commun. 473: 947-952. PMID: 27040770
8. Lack of skeletal muscle IL-6 influences hepatic glucose metabolism in mice during prolonged exercise.  |  Bertholdt, L., et al. 2017. Am J Physiol Regul Integr Comp Physiol. 312: R626-R636. PMID: 28122718
9. Regulation of interleukin-6 secretion from mononuclear blood cells by extracellular calcium.  |  Bornefalk, E., et al. 1997. J Bone Miner Res. 12: 228-33. PMID: 9041054
10. Nociceptive responses in interleukin-6-deficient mice to peripheral inflammation and peripheral nerve section.  |  Xu, XJ., et al. 1997. Cytokine. 9: 1028-33. PMID: 9417815

The preferred IL-6 antibody is IL-6 Antibody (E-4): sc-28343.