TRIM35 Antibody (31-M): sc-100880

TRIM35 Antibody (31-M) is a mouse monoclonal IgG2a kappa light chain antibody that detects TRIM35 protein of human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry with paraffin-embedded sections (IHCP), and enzyme-linked immunosorbent assay (ELISA). TRIM35 (31-M) antibody is available as non-conjugated anti-TRIM35 antibody. TRIM35, also known as tripartite motif-containing 35, HLS5 (hemopoietic lineage switch protein 5), or MAIR (macrophage-derived apoptosis-inducing RBCC protein), is a 493 amino acid protein that plays a crucial role in cellular processes, particularly in apoptosis regulation and tumor suppression. TRIM35 is characterized by a unique structure, including a B box-type zinc finger, a coiled-coil domain, a SPRY domain, and a RING-type zinc finger, all contributing to mediating protein-protein and protein-DNA interactions. These domains facilitate TRIM35′s involvement in various signaling pathways and cellular responses, particularly in cancer biology. TRIM35 is localized to cytoplasmic granules and punctate nuclear bodies, indicating active participation in cellular signaling and stress responses. Forced expression of TRIM35 in HeLa cells inhibits tumorigenicity, cell growth, and clonogenicity, suggesting potential function as a tumor suppressor. The gene encoding TRIM35 is located on chromosome 8, a region associated with several leukemias and solid tumors, underscoring importance in cancer research and therapeutic development.

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TRIM35 Antibody (31-M) References:

1. The tripartite motif family identifies cell compartments.  |  Reymond, A., et al. 2001. EMBO J. 20: 2140-51. PMID: 11331580
2. Cloning and characterization of a novel RING-B-box-coiled-coil protein with apoptotic function.  |  Kimura, F., et al. 2003. J Biol Chem. 278: 25046-54. PMID: 12692137
3. HLS5, a novel RBCC (ring finger, B box, coiled-coil) family member isolated from a hemopoietic lineage switch, is a candidate tumor suppressor.  |  Lalonde, JP., et al. 2004. J Biol Chem. 279: 8181-9. PMID: 14662771
4. The RING-finger protein haprin: domains and function in the acrosome reaction.  |  Kitamura, K., et al. 2005. Curr Protein Pept Sci. 6: 567-74. PMID: 16381605
5. Subclassification of the RBCC/TRIM superfamily reveals a novel motif necessary for microtubule binding.  |  Short, KM. and Cox, TC. 2006. J Biol Chem. 281: 8970-80. PMID: 16434393
6. Structure-based mutagenesis of the substrate-recognition domain of Nrdp1/FLRF identifies the binding site for the receptor tyrosine kinase ErbB3.  |  Bouyain, S. and Leahy, DJ. 2007. Protein Sci. 16: 654-61. PMID: 17384230
7. Structure of the MID1 tandem B-boxes reveals an interaction reminiscent of intermolecular ring heterodimers.  |  Tao, H., et al. 2008. Biochemistry. 47: 2450-7. PMID: 18220417

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-TRIM35 Antibody (31-M) has 1 citations in a variety of scientific publications.

Primary antibodies like Anti-TRIM35 Antibody (31-M) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

TRIM35 Antibody (31-M) Specifications

Alternate Product Name: TRIM35

Product Clone Name: 31-M

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG_2a kappa light chain

Amino Acids: 394-494

Epitope Target: TRIM35

Epitope Species: human

Product Reactivity/Detection: TRIM35

Species Reactivity/Detection: human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), ELISA

Vialing Information: Each vial contains 100 µg IgG_2a kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 1 citations in various scientific publications

Available Conjugates/Forms for TRIM35 Antibody (31-M): N/A