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ATF3 Antibody - BSA Free

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Western Blot: ATF3 Antibody [NBP1-02935] - Western blot analysis of ATF3 on UV treated NIH/3T3 cell extracts using NBP1-02935.
Western Blot: ATF3 Antibody [NBP1-02935] - Western blot analysis of ATF3 on UV treated NIH/3T3 cell extracts using NBP1-02935.

Product Details

Summary
Reactivity Hu, Mu, Rt, MaSpecies Glossary
Applications WB, ELISA, IHC
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Format
BSA Free
Concentration
1.27 mg/ml

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ATF3 Antibody - BSA Free Summary

Immunogen
A synthetic peptide corresponding to a region of human ATF3 (within residues 100-150). [UniProt# P18847]
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
ATF3
Purity
Immunogen affinity purified
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Applications/Dilutions

Dilutions
  • ELISA 1:100-1:2000
  • Immunohistochemistry 5 ug/ml
  • Immunohistochemistry-Paraffin 5 ug/ml
  • Western Blot 1:200
Application Notes
This ATF3 antibody is useful for Western Blot, ELISA and Immunohistochemistry paraffin embedded sections.
Theoretical MW
17 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Control Peptide
ATF3 Peptide (NBP1-02935PEP)
test
Publications
Read Publications using
NBP1-02935 in the following applications:

Reactivity Notes

Predicted to react with most mammalian species based on sequence homology.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS and 30% Glycerol
Preservative
0.1% Sodium Azide
Concentration
1.27 mg/ml
Purity
Immunogen affinity purified

Alternate Names for ATF3 Antibody - BSA Free

  • activating transcription factor 3cAMP-dependent transcription factor ATF-3
  • ATF3deltaZip2
  • ATF3deltaZip2c
  • ATF3deltaZip3
  • cyclic AMP-dependent transcription factor ATF-3

Background

ATF3 (activating transcription factor 3) belongs to ATF/CREB family of transcription factors and it binds Cre 5'-GTGACGT[AC][AG]-3' a sequence which is present in several cellular as well as viral promoters. ATF3 exerts its repressive effects on transcription from promoters with ATF sites by stabilizing the binding of inhibitory cofactors of target promoters. Expressed by various cell types with nuclear localization, ATF3 expression gets upregulated by a variety of extracellular stimuli such as cytokines, chemokines, growth factors, hormones, hypoxia, DNA damage, ER stress and starvation. Originally considered a stress-response gene, ATF3 now has emerged as a factor implicated in general adaptive responses such as environmental, emotional and nutritional alterations. Its disorders have been associated with inflammatory and immune diseases as well as cancer, and unlike other ATF/CREB family members, ATF3 is emerging as a factor involved in protection against malignant cells and invading pathogens.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Phone: 1-888-506-6887
Phone: 303-730-1950
Fax: 303-730-1966
Email: novus@novusbio.com
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Product PDFs

  NBP1-02935 Specific Protocol
  SDS
  PDF Datasheet
  Request CofA

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Bioinformatics

Gene Symbol ATF3
Entrez
Uniprot