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WBP2 Antibody (D-12): sc-514247

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Datasheets
  • WBP2 Antibody (D-12) is a mouse monoclonal IgG1 λ WBP2 antibody, cited in 2 publications, provided at 200 µg/ml
  • specific for an epitope mapping between amino acids 129-150 within an internal region of WBP2 of human origin
  • WBP2 Antibody (D-12) is recommended for detection of WBP2 of mouse, rat and human origin by WB, IP, IF and ELISA
  • Anti-WBP2 Antibody (D-12) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
  • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
  • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
  • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of WBP2 (D-12): sc-514247.
  • m-IgGλ BP-HRP (mouse IgGλ binding protein-HRP) is the preferred secondary detection reagent for WBP2 Antibody (D-12) for WB applications. This reagent is now offered in a bundle with WBP2 Antibody (D-12) (see ordering information below). For additional m-IgGλ BP conjugates see our complete list of Mouse IgG Binding Proteins.

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WBP2 Antibody (D-12) is an IgG1 λ mouse monoclonal WBP2 antibody (also designated WBP2 antibody) that detects the WBP2 protein of mouse, rat and human origin by WB, IP, IF and ELISA. WBP2 Antibody (D-12) is available as both the non-conjugated anti-WBP2 antibody form, as well as multiple conjugated forms of anti-WBP2 antibody, including agarose, HRP, PE, FITC and multiple Alexa Fluor® conjugates. WW domain-binding protein 2 (WBP2) is a 261 amino acid protein expressed in most tissues. The WW domain is composed of 38 to 40 semi-conserved amino acids and is shared by various groups of proteins, including structural, regulatory and signaling proteins. The domain mediates protein-protein interactions through the binding of polyproline ligands. WBP2 binds to the WW domain of Yes-associated protein (YAP), WW domain containing E3 ubiquitin protein ligase 1 (AIP5) and WW domain containing E3 ubiquitin protein ligase 2 (AIP2). The gene encoding WBP2 is located on human chromosome 17, which comprises over 2.5% of the human genome and encodes over 1,200 genes, some of which are involved in tumor suppression and in the pathogenesis of Li-Fraumeni syndrome, early onset breast cancer and a predisposition to cancers of the ovary, colon, prostate gland and fallopian tubes.

For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.

Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA

LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences

WBP2 Antibody (D-12) References:

  1. Identification of the WW domain-interaction sites in the unstructured N-terminal domain of EBV LMP 2A.  |  Seo, MD., et al. 2007. FEBS Lett. 581: 65-70. PMID: 17174309
  2. Nedd4-2 isoforms ubiquitinate individual epithelial sodium channel subunits and reduce surface expression and function of the epithelial sodium channel.  |  Raikwar, NS. and Thomas, CP. 2008. Am J Physiol Renal Physiol. 294: F1157-65. PMID: 18322022
  3. Identification and structural mechanism for a novel interaction between a ubiquitin ligase WWP1 and Nogo-A, a key inhibitor for central nervous system regeneration.  |  Qin, H., et al. 2008. Biochemistry. 47: 13647-58. PMID: 19035836
  4. The E3 ubiquitin ligase WWP1 selectively targets HER4 and its proteolytically derived signaling isoforms for degradation.  |  Feng, SM., et al. 2009. Mol Cell Biol. 29: 892-906. PMID: 19047365
  5. YAP, TAZ, and Yorkie: a conserved family of signal-responsive transcriptional coregulators in animal development and human disease.  |  Wang, K., et al. 2009. Biochem Cell Biol. 87: 77-91. PMID: 19234525
  6. The WW domain of Yes-associated protein binds a proline-rich ligand that differs from the consensus established for Src homology 3-binding modules.  |  Chen, HI. and Sudol, M. 1995. Proc Natl Acad Sci U S A. 92: 7819-23. PMID: 7644498
  7. Identification of novel human WW domain-containing proteins by cloning of ligand targets.  |  Pirozzi, G., et al. 1997. J Biol Chem. 272: 14611-6. PMID: 9169421
  8. Characterization of the WW domain of human yes-associated protein and its polyproline-containing ligands.  |  Chen, HI., et al. 1997. J Biol Chem. 272: 17070-7. PMID: 9202023

Ordering Information

Product NameCatalog #UNITPriceQtyFAVORITES

WBP2 Antibody (D-12)

sc-514247
200 µg/ml
$316.00

WBP2 Antibody (D-12): m-IgGλ BP-HRP Bundle

sc-524663
200 µg Ab, 40 µg BP
$354.00

WBP2 Antibody (D-12) AC

sc-514247 AC
500 µg/ml, 25% agarose
$416.00

WBP2 Antibody (D-12) HRP

sc-514247 HRP
200 µg/ml
$316.00

WBP2 Antibody (D-12) FITC

sc-514247 FITC
200 µg/ml
$330.00

WBP2 Antibody (D-12) PE

sc-514247 PE
200 µg/ml
$343.00

WBP2 Antibody (D-12) Alexa Fluor® 488

sc-514247 AF488
200 µg/ml
$357.00

WBP2 Antibody (D-12) Alexa Fluor® 546

sc-514247 AF546
200 µg/ml
$357.00

WBP2 Antibody (D-12) Alexa Fluor® 594

sc-514247 AF594
200 µg/ml
$357.00

WBP2 Antibody (D-12) Alexa Fluor® 647

sc-514247 AF647
200 µg/ml
$357.00

WBP2 Antibody (D-12) Alexa Fluor® 680

sc-514247 AF680
200 µg/ml
$357.00

WBP2 Antibody (D-12) Alexa Fluor® 790

sc-514247 AF790
200 µg/ml
$357.00

WBP2 (D-12) Neutralizing Peptide

sc-514247 P
100 µg/0.5 ml
$68.00

What application is the blocking peptide sc-514247 P appropriate for?

Asked by: jerojero
Thank you for your question. The blocking peptide is intended for use as a negative control, by pre-adsorbing the mouse monoclonal antibody against the antigen. For full protocol details, please contact our Technical Services Department or view our online protocol here: https://www.scbt.com/scbt/resources/protocols/peptide-neutralization
Answered by: Technical Support
Date published: 2017-03-01
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Rated 4 out of 5 by from good antibodyI used this antibody for DuoLink PLA, and also for WB. For DuoLink PLA, it is very good. it is ok for WB at concentration of 1:500; however, there are 2 bands and it is hard to know which band is the right band, since I am doing siRNA. From siRNA WB, one band seems to have much lower in the intensity after the knockdown. I would assume that is the right band.
Date published: 2019-03-01
Rated 5 out of 5 by from Good Western blot data of WBP2 expression in nonGood Western blot data of WBP2 expression in non-transfected and human WBP2 transfected 293 whole cell lysates. -SCBT QC
Date published: 2014-02-26
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WBP2 Antibody (D-12) is rated 4.5 out of 5 by 2.
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