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ANAPC2 Antibody (OTI1A6)

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Western Blot: ANAPC2 Antibody (1A6) [NBP2-01128] Analysis of extracts (35ug) from 9 different cell lines by using anti-ANAPC2 monoclonal antibody.

Product Details

Summary
Reactivity Hu, Mu, Rt, PmSpecies Glossary
Applications WB, Flow, ICC/IF, IHC
Clone
OTI1A6
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
0.74 mg/ml

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ANAPC2 Antibody (OTI1A6) Summary

Immunogen
Full length human recombinant protein of human ANAPC2(NP_037498) produced in HEk293 cell.
Isotype
IgG2a
Clonality
Monoclonal
Host
Mouse
Gene
ANAPC2
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Flow Cytometry 1:100
  • Immunocytochemistry/ Immunofluorescence 1:100
  • Immunohistochemistry 1:150
  • Immunohistochemistry-Paraffin 1:150
  • Western Blot 1:500-2000
Theoretical MW
93.6 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.3), 1.0% BSA and 50% Glycerol
Preservative
0.02% Sodium Azide
Concentration
0.74 mg/ml
Purity
Immunogen affinity purified

Alternate Names for ANAPC2 Antibody (OTI1A6)

  • anaphase promoting complex subunit 2
  • anaphase-promoting complex subunit 2
  • APC2KIAA1406Cyclosome subunit 2
  • RP11-350O14.5

Background

A large protein complex, termed the anaphase-promoting complex (APC), or the cyclosome, promotes metaphase-anaphase transition by ubiquitinating its specific substrates such as mitotic cyclins and anaphase inhibitor, which are subsequently degraded by the 26S proteasome. Biochemical studies have shown that the vertebrate APC contains eight subunits. The composition of the APC is highly conserved in organisms from yeast to humans. The product of this gene is a component of the complex and shares sequence similarity with a recently identified family of proteins called cullins, which may also be involved in ubiquitin-mediated degradation. [provided by RefSeq].

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Bioinformatics

Gene Symbol ANAPC2
Entrez