PHKA1 Antibody - BSA Free Summary
| Immunogen |
This antibody was developed against Recombinant Protein corresponding to amino acids: AHSLRCSAEEATEGLMNLSPSAMKNLLHHILSGKEFGVERSVRPTDSNVSPAISIHEIGAVGATKTERTGIMQLKSEIKQSPGTSMTPSSGSFPSAYDQQSSKDSRQGQWQ |
| Predicted Species |
Mouse (90%). Backed by our 100% Guarantee. |
| Isotype |
IgG |
| Clonality |
Polyclonal |
| Host |
Rabbit |
| Gene |
PHKA1 |
| Purity |
Immunogen affinity purified |
| Innovator's Reward |
Test in a species/application not listed above to receive a full credit towards a future purchase. |
Applications/Dilutions
| Dilutions |
- Immunocytochemistry/ Immunofluorescence 0.25-2 ug/ml
- Immunohistochemistry 1:50 - 1:200
- Immunohistochemistry-Paraffin 1:50 - 1:200
- Western Blot 0.04-0.4 ug/ml
|
| Application Notes |
For IHC-Paraffin, HIER pH 6 retrieval is recommended. Immunocytochemistry/Immunofluorescence Fixation Permeabilization: Use PFA/Triton X-100. |
| Control Peptide |
|
Reactivity Notes
Immunogen displays the following percentage of sequence identity for non-tested species: Rat (80%)
Packaging, Storage & Formulations
| Storage |
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
| Buffer |
PBS (pH 7.2) and 40% Glycerol |
| Preservative |
0.02% Sodium Azide |
| Purity |
Immunogen affinity purified |
Alternate Names for PHKA1 Antibody - BSA Free
Background
The PHKA1 gene encodes the alpha subunit of muscle phosphorylase kinase (EC 2.7.1.38), a key regulatory enzyme of glycogen metabolism. Phosphorylase kinase consists of 4 copies of an alpha-beta-gamma-delta tetramer. The alpha, beta (PHKB; MIM 172490), and gamma (PHKG1; MIM 172470 and PHKG2; MIM 172471) subunits have several isoforms; the delta subunit is calmodulin (CALM1; MIM 114180). PHKA2 (MIM 306000) encodes the alpha subunit of liver-specific phosphorylase kinase and is also located on the X chromosome.[supplied by OMIM]
Limitations
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are
guaranteed for 1 year from date of receipt.
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Product General Protocols
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FAQs for PHKA1 Antibody (NBP1-87281) (0)
Secondary Antibodies
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Isotype Controls
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Blogs on PHKA1
![Western Blot: PHKA1 Antibody [NBP1-87281] - Lane 1: Marker [kDa] 219, 112, 85, 49, 32, 25, 18. Lane 2: Human cell line RT-4. Lane 3: Human cell line U-251MG sp. Lane 4: Human cell line A-431. Lane 5: Human liver tissue. Lane 6: Human tonsil tissue](http://images.novusbio.com/fullsize/PHKA1-Antibody-Western-Blot-NBP1-87281-img0004.jpg "Western Blot: PHKA1 Antibody [NBP1-87281] - Lane 1: Marker [kDa] 219, 112, 85, 49, 32, 25, 18. Lane 2: Human cell line RT-4. Lane 3: Human cell line U-251MG sp. Lane 4: Human cell line A-431. Lane 5: Human liver tissue. Lane 6: Human tonsil tissue")
![Immunocytochemistry/Immunofluorescence: PHKA1 Antibody [NBP1-87281] - Immunofluorescent staining of human cell line A-431 shows localization to vesicles.](http://images.novusbio.com/fullsize/PHKA1-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-87281-img0005.jpg "Immunocytochemistry/Immunofluorescence: PHKA1 Antibody [NBP1-87281] - Immunofluorescent staining of human cell line A-431 shows localization to vesicles.")
![Immunohistochemistry-Paraffin: PHKA1 Antibody [NBP1-87281] - Staining of human small intestine shows low expression as expected.](http://images.novusbio.com/fullsize/PHKA1-Antibody-Immunohistochemistry-Paraffin-NBP1-87281-img0008.jpg "Immunohistochemistry-Paraffin: PHKA1 Antibody [NBP1-87281] - Staining of human small intestine shows low expression as expected.")
![Immunohistochemistry-Paraffin: PHKA1 Antibody [NBP1-87281] - Staining of human pancreas shows strong granular cytoplasmic positivity in exocrine glandular cells.](http://images.novusbio.com/fullsize/PHKA1-Antibody-Immunohistochemistry-Paraffin-NBP1-87281-img0006.jpg "Immunohistochemistry-Paraffin: PHKA1 Antibody [NBP1-87281] - Staining of human pancreas shows strong granular cytoplasmic positivity in exocrine glandular cells.")
![Immunohistochemistry-Paraffin: PHKA1 Antibody [NBP1-87281] - Staining of human parathyroid gland shows high expression.](http://images.novusbio.com/fullsize/PHKA1-Antibody-Immunohistochemistry-Paraffin-NBP1-87281-img0007.jpg "Immunohistochemistry-Paraffin: PHKA1 Antibody [NBP1-87281] - Staining of human parathyroid gland shows high expression.")
![Immunohistochemistry-Paraffin: PHKA1 Antibody [NBP1-87281] - Staining in human parathyroid gland and small intestine tissues using anti-PHKA1 antibody. Corresponding PHKA1 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/PHKA1-Antibody-Immunohistochemistry-Paraffin-NBP1-87281-img0009.jpg "Immunohistochemistry-Paraffin: PHKA1 Antibody [NBP1-87281] - Staining in human parathyroid gland and small intestine tissues using anti-PHKA1 antibody. Corresponding PHKA1 RNA-seq data are presented for the same tissues.")
![Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).](https://images.novusbio.com/images/Goat-anti-Rabbit-IgG-H+L-Secondary-Antibody-HRP-Western-Blot-NB7160-img0001.jpg "Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).")
![Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.](https://images.novusbio.com/images/Rabbit--Mouse-IgG-Isotype-Control-Flow-Cytometry-NBP2-24891-img0001.jpg "Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.")