Ribophorin II Antibody - BSA Free Summary
| Immunogen |
The immunogen this antibody was made to, maps to a region between residue 350 to 400 of human Ribophorin II using the numbering given in entry NP_002942.2 (GeneID 6185). |
| Isotype |
IgG |
| Clonality |
Polyclonal |
| Host |
Rabbit |
| Gene |
RPN2 |
| Purity |
Immunogen affinity purified |
| Innovator's Reward |
Test in a species/application not listed above to receive a full credit towards a future purchase. |
Applications/Dilutions
| Dilutions |
- Immunohistochemistry
- Immunoprecipitation 2 - 10 ug/mg lysate
- Western Blot 1:1000 - 1:5000
|
Packaging, Storage & Formulations
| Storage |
Store at 4C. Do not freeze. |
| Buffer |
Tris-Citrate/Phosphate (pH 7.0 - 8.0) |
| Preservative |
0.09% Sodium Azide |
| Concentration |
1.0 mg/ml |
| Purity |
Immunogen affinity purified |
Alternate Names for Ribophorin II Antibody - BSA Free
Background
Ribophorin II encodes a type I integral membrane protein found only in the rough endoplasmic reticulum. The encoded protein is part of an N-oligosaccharyl transferase complex that links high mannose oligosaccharides to asparagine residues found in the Asn-X-Ser/Thr consensus motif of nascent polypeptide chains. This protein is similar in sequence to the yeast oligosaccharyl transferase subunit SWP1.
Limitations
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are
guaranteed for 1 year from date of receipt.
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Publications for Ribophorin II Antibody (NBP2-32178) (0)
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Product General Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
Video Protocols
FAQs for Ribophorin II Antibody (NBP2-32178) (0)
Secondary Antibodies
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Isotype Controls
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Additional Ribophorin II Products
Blogs on Ribophorin II
![Western Blot: Ribophorin II Antibody [NBP2-32178] - Samples: Whole cell lysate (50 ug) from HeLa, 293T, and Jurkat cells. Antibodies: Affinity purified rabbit anti-Ribophorin II antibody NBP2-32178 used for WB at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.](http://images.novusbio.com/fullsize/Ribophorin-II-Antibody-Western-Blot-NBP2-32178-img0001.jpg "Western Blot: Ribophorin II Antibody [NBP2-32178] - Samples: Whole cell lysate (50 ug) from HeLa, 293T, and Jurkat cells. Antibodies: Affinity purified rabbit anti-Ribophorin II antibody NBP2-32178 used for WB at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.")
![Immunohistochemistry: Ribophorin II Antibody [NBP2-32178] - Sample: FFPE section of human prostate carcinoma. Antibody: Affinity purified rabbit anti- RPN2 used at a dilution of 1:1,000 (1ug/ml). Detection: DAB. Counterstain: IHC Hematoxylin (blue).](http://images.novusbio.com/fullsize/Ribophorin-II-Antibody-Immunohistochemistry-NBP2-32178-img0004.jpg "Immunohistochemistry: Ribophorin II Antibody [NBP2-32178] - Sample: FFPE section of human prostate carcinoma. Antibody: Affinity purified rabbit anti- RPN2 used at a dilution of 1:1,000 (1ug/ml). Detection: DAB. Counterstain: IHC Hematoxylin (blue).")
![Immunohistochemistry: Ribophorin II Antibody [NBP2-32178] - Sample: FFPE section of human mammary glands. Antibody: Affinity purified rabbit anti- RPN2 used at a dilution of 1:1,000 (1ug/ml). Detection: DAB. Counterstain: IHC Hematoxylin (blue).](http://images.novusbio.com/fullsize/Ribophorin-II-Antibody-Immunohistochemistry-NBP2-32178-img0003.jpg "Immunohistochemistry: Ribophorin II Antibody [NBP2-32178] - Sample: FFPE section of human mammary glands. Antibody: Affinity purified rabbit anti- RPN2 used at a dilution of 1:1,000 (1ug/ml). Detection: DAB. Counterstain: IHC Hematoxylin (blue).")
![Immunoprecipitation: Ribophorin II Antibody [NBP2-32178] - Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-Ribophorin II antibody NBP2-32178 used for IP at 6 ug per reaction. Ribophorin II was also immunoprecipitated by rabbit anti-Ribophorin II antibody BL15802. For blotting immunoprecipitated Ribophorin II, NBP2-32178 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.](http://images.novusbio.com/fullsize/Ribophorin-II-Antibody-Immunoprecipitation-NBP2-32178-img0002.jpg "Immunoprecipitation: Ribophorin II Antibody [NBP2-32178] - Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-Ribophorin II antibody NBP2-32178 used for IP at 6 ug per reaction. Ribophorin II was also immunoprecipitated by rabbit anti-Ribophorin II antibody BL15802. For blotting immunoprecipitated Ribophorin II, NBP2-32178 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.")
![Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).](https://images.novusbio.com/images/Goat-anti-Rabbit-IgG-H+L-Secondary-Antibody-HRP-Western-Blot-NB7160-img0001.jpg "Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).")
![Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.](https://images.novusbio.com/images/Rabbit--Mouse-IgG-Isotype-Control-Flow-Cytometry-NBP2-24891-img0001.jpg "Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.")