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Phospho-Elk-1 (Ser383) (2B1) Mouse mAb

Phospho-Elk-1 (Ser383) (2B1) Mouse mAb #5160

Inquiry Info. # 5160

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Supporting Data

REACTIVITY
SENSITIVITY
MW (kDa)	62
Source/Isotype	Mouse

Product Information

Storage

Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

Specificity / Sensitivity

Phospho-Elk-1 (Ser383) (2B1) Mouse mAb can be used in high throughput kinase assays and drug discovery applications. It detects endogenous levels of Elk-1 protein only when phosphorylated at Ser383.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic phosphopeptide corresponding to residues around Ser383 of human Elk-1.

Background

The ETS transcription factor Elk-1 is a component of the ternary complex that binds the serum response element (SRE) and mediates gene activity in response to serum and growth factors (1-3). Elk-1 is phosphorylated by MAP kinase pathways at a cluster of S/T motifs at its carboxy terminus; phosphorylation at these sites, particularly Ser383, is critical for transcriptional activation by Elk-1. Elk-1 appears to be a direct target of activated MAP kinase: (a) biochemical studies indicate that Elk-1 is a good substrate for MAP kinase; (b) the kinetics of Elk-1 phosphorylation and activation correlate with MAP kinase activity; (c) interfering mutants of MAP kinase block Elk-1 activation in vivo. Other studies have shown that Elk-1 (Ser383) is also a target of the stress-activated kinase SAPK/JNK (4,5).

Description: Cell Signaling Technology antibody formulated in PBS (no BSA/no glycerol) and quality controlled for use in ELISA and other drug discovery applications. This is a sample antibody and intended for use by drug discovery scientists. Please inquire for bulk pricing of this antibody.

Database Links

UniProt ID: P19419

Entrez-Gene Id: 2002

Limited Uses

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For Research Use Only. Not For Use In Diagnostic Procedures.

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