MHC class I Antibody (F-3): sc-55582

MHC class I Antibody (F-3) is a mouse monoclonal IgG2a kappa light chain antibody, designed to recognize Major Histocompatibility Complex class I (MHC I) proteins, also known as Human Leukocyte Antigen (HLA) class I molecules, including HLA-A, HLA-B, and HLA-C, in human samples. Anti-MHC class I antibody (F-3) targets an epitope spanning amino acids 63-362 of human MHC class I, ensuring precise and reliable binding. MHC class I proteins are crucial cell-surface receptors that present endogenous peptides to cytotoxic T lymphocytes, a fundamental process for immune surveillance and the elimination of infected or malignant cells. By facilitating the presentation of antigenic peptides, MHC class I molecules play a vital role in activating and regulating the immune response, making accurate detection essential for both immunological research and clinical diagnostics. MHC class I (F-3) monoclonal antibody is validated for use in western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry with paraffin-embedded sections (IHCP), and enzyme-linked immunosorbent assay (ELISA), offering versatility for diverse experimental needs. Available in both conjugated and unconjugated forms, including conjugates with agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® dyes, MHC class I (F-3) antibody accommodates different detection systems and protocols. By enabling detailed analysis of MHC class I expression and function, MHC class I (F-3) monoclonal antibody serves as an invaluable tool for advancing research in immunology, oncology, and related biomedical fields, ultimately contributing to a deeper understanding of immune mechanisms and the development of targeted therapies.

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Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-MHC class I Antibody (F-3) has 22 citations in a variety of scientific publications.

Primary antibodies like Anti-MHC class I Antibody (F-3) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

MHC class I Antibody (F-3) Specifications

Alternate Product Name: Human Leukocyte Antigen (HLA) HLA-A, HLA-B, HLA-C class 1 heavy chain receptor

Product Clone Name: F-3

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG_2a kappa light chain

Amino Acids: 63-362

Epitope Target: MHC class I

Epitope Species: human

Product Reactivity/Detection: MHC class I

Species Reactivity/Detection: human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), ELISA

Vialing Information: Each vial contains 200 µg IgG_2a kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 22 citations in various scientific publications

Available Conjugates/Forms for MHC class I Antibody (F-3): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin

The preferred MHC class I antibody is MHC class I Antibody (W6/32): sc-32235.