Human/Mouse/Rat Plexin A2 Antibody

Catalog # Availability Size / Price Qty
AF5486-SP Ships in 5-7 Days25 ug / $149
AF5486 In Stock100 ug / $469
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Detection of Mouse and Rat Plexin A2 by Western Blot.
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Product Details
Citations (5)
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Reviews (1)

Human/Mouse/Rat Plexin A2 Antibody Summary

Species Reactivity
Human, Mouse, Rat
Specificity
Detects mouse and rat Plexin A2 in direct ELISAs and Western blots. In direct ELISAs, less than 1% cross-reactivity with recombinant mouse (rm) Plexin A1, rmPlexin A3, and recombinant human Plexin B1 is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
Chinese hamster ovary cell line CHO-derived recombinant mouse Plexin A2
Met35-Pro1237
Accession # P70207
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
See below
Simple Western
10 µg/mL
See below
Flow Cytometry
2.5 µg/106 cells
See below
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
 
Immunocytochemistry
10 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Mouse and Rat Plexin A2 antibody by Western Blot. View Larger

Detection of Mouse and Rat Plexin A2 by Western Blot. Western blot shows lysates of bEnd.3 mouse endothelioma cell line and rat embryonic cortical neuron cells. PVDF Membrane was probed with 1 µg/mL of Goat Anti-Human/Mouse/Rat Plexin A2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5486) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for Plexin A2 at approximately 210 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.

Flow Cytometry Detection of Plexin A2 antibody in bEnd.3 Mouse Cell Line antibody by Flow Cytometry. View Larger

Detection of Plexin A2 in bEnd.3 Mouse Cell Line by Flow Cytometry. bEnd.3 mouse endothelioma cell line was stained with Goat Anti-Human/Mouse/Rat Plexin A2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5486, filled histogram) or isotype control antibody (Catalog # AB-108-C, open histogram), followed by Allophycocyanin-conjugated Anti-Goat IgG Secondary Antibody (Catalog # F0108).

Immunocytochemistry Plexin A2 antibody in bEnd by Immunocytochemistry (ICC).3 Mouse Cell Line by Immunocytochemistry (ICC). View Larger

Plexin A2 in bEnd.3 Mouse Cell Line. Plexin A2 was detected in immersion fixed bEnd.3 mouse endothelioma cell line using Goat Anti-Human/Mouse/Rat Plexin A2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5486) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to cell surfaces and cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Immunocytochemistry Plexin A2 antibody in HUVEC Human Cells by Immunocytochemistry (ICC). View Larger

Plexin A2 in HUVEC Human Cells. Plexin A2 was detected in immersion fixed HUVEC human umbilical vein endothelial cells using Goat Anti-Human/Mouse/Rat Plexin A2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5486) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to cell surfaces and cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Simple Western Detection of Mouse Plexin A2 antibody by Simple Western<sup>TM</sup>. View Larger

Detection of Mouse Plexin A2 by Simple WesternTM. Simple Western lane view shows lysates of bEnd.3 mouse endothelioma cell line, loaded at 0.2 mg/mL. A specific band was detected for Plexin A2 at approximately 246 kDa (as indicated) using 10 µg/mL of Goat Anti-Human/Mouse/Rat Plexin A2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5486) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the 66-440 kDa separation system.

Reconstitution Calculator

Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
In Stock
2 mL / $12
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Plexin A2

Plexin A2 (also OCT) is a 220-230 kDa member of the plexin-A subfamily, plexin family of proteins. It is found on cerebellar granule cells, sensory neurons, and cardiac neural crest cells. It participates in cell migration and axon guidance, and does so by serving as a receptor for Sema6A and B, and as a coreceptor with neuropilin-1 for Sema3A and C. Mature mouse Plexin A2 is an 1860 amino acid (aa) type I transmembrane glycoprotein. It contains a 1203 aa extracellular domain (ECD) and a 636 aa cytoplasmic region. The ECD contains one Sema domain (aa 35-508), three PSI domains (aa 510-855) and four IPT regions (aa 858-1228). There is one alternate start site 10 aa upstream of the standard start site. Over aa 35-1237, mouse Plexin A2 shares 96% aa sequence identity with human Plexin A2.

Entrez Gene IDs
5362 (Human); 18845 (Mouse); 289392 (Rat)
Alternate Names
FLJ11751; FLJ30634; KIAA0463plexin 2; OCT; OCTSemaphorin receptor OCT; Plexin A2; PLXN2; PLXN2plexin-A2; PLXNA2; transmembrane protein OCT

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Citations for Human/Mouse/Rat Plexin A2 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

5 Citations: Showing 1 - 5
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  1. Semmaphorin 3?A causes immune suppression by inducing cytoskeletal paralysis in tumour-specific CD8+ T cells
    Authors: Barnkob, MB;Michaels, YS;André, V;Macklin, PS;Gileadi, U;Valvo, S;Rei, M;Kulicke, C;Chen, JL;Jain, V;Woodcock, VK;Colin-York, H;Hadjinicolaou, AV;Kong, Y;Mayya, V;Mazet, JM;Mead, GJ;Bull, JA;Rijal, P;Pugh, CW;Townsend, AR;Gérard, A;Olsen, LR;Fritzsche, M;Fulga, TA;Dustin, ML;Jones, EY;Cerundolo, V;
    Nature communications
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  2. SEMA6A drives GnRH neuron-dependent puberty onset by tuning median eminence vascular permeability
    Authors: Lettieri, A;Oleari, R;van den Munkhof, MH;van Battum, EY;Verhagen, MG;Tacconi, C;Spreafico, M;Paganoni, AJJ;Azzarelli, R;Andre', V;Amoruso, F;Palazzolo, L;Eberini, I;Dunkel, L;Howard, SR;Fantin, A;Pasterkamp, RJ;Cariboni, A;
    Nature communications
    Species: Mouse, Transgenic Mouse
    Sample Types: Whole Cells, Whole Tissue
    Applications: Immunohistochemistry, Immunocytochemistry
  3. Semaphorin3A/PlexinA3 association with the Scribble scaffold for cGMP increase is required for apical dendrite development
    Authors: J Szczurkows, A Guo, J Martin, SI Lee, E Martinez, CT Chien, TA Khan, R Singh, D Dadson, TS Tran, S Pautot, M Shelly
    Cell Reports, 2022-03-15;38(11):110483.
    Species: Mouse, Rat
    Sample Types: Whole Cells
    Applications: ICC, IHC
  4. Sympathetic nerve repulsion inhibited by designer molecules in vitro and role in experimental arthritis
    Authors: Rainer H Straub
    J. Med. Microbiol., 2016-11-14;0(0):.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Neutralization
  5. Neural crest-derived SEMA3C activates endothelial NRP1 for cardiac outflow tract septation.
    Authors: Plein A, Calmont A, Fantin A, Denti L, Anderson N, Scambler P, Ruhrberg C
    J Clin Invest, 2015-06-08;125(7):2661-76.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC

FAQs

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Isotype Controls

Reconstitution Buffers

Secondary Antibodies

Goat IgG (H+L) Antibody

WB,Secondary

Reviews for Human/Mouse/Rat Plexin A2 Antibody

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Human/Mouse/Rat Plexin A2 Antibody
By Anonymous on 03/29/2018
Application: WB Sample Tested: Zebrafish Species: Zebrafish

Western blot on zebrafish embryo extracts

Customer is aware this antibody is not validated with Zebrafish. Provided review as help to others.