Human/Mouse/Rat Plexin A2 Antibody Summary
Met35-Pro1237
Accession # P70207
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Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
Detection of Mouse and Rat Plexin A2 by Western Blot. Western blot shows lysates of bEnd.3 mouse endothelioma cell line and rat embryonic cortical neuron cells. PVDF Membrane was probed with 1 µg/mL of Goat Anti-Human/Mouse/Rat Plexin A2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5486) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for Plexin A2 at approximately 210 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.
Detection of Plexin A2 in bEnd.3 Mouse Cell Line by Flow Cytometry. bEnd.3 mouse endothelioma cell line was stained with Goat Anti-Human/Mouse/Rat Plexin A2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5486, filled histogram) or isotype control antibody (Catalog # AB-108-C, open histogram), followed by Allophycocyanin-conjugated Anti-Goat IgG Secondary Antibody (Catalog # F0108).
Plexin A2 in bEnd.3 Mouse Cell Line. Plexin A2 was detected in immersion fixed bEnd.3 mouse endothelioma cell line using Goat Anti-Human/Mouse/Rat Plexin A2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5486) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to cell surfaces and cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Plexin A2 in HUVEC Human Cells. Plexin A2 was detected in immersion fixed HUVEC human umbilical vein endothelial cells using Goat Anti-Human/Mouse/Rat Plexin A2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5486) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to cell surfaces and cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Detection of Mouse Plexin A2 by Simple WesternTM. Simple Western lane view shows lysates of bEnd.3 mouse endothelioma cell line, loaded at 0.2 mg/mL. A specific band was detected for Plexin A2 at approximately 246 kDa (as indicated) using 10 µg/mL of Goat Anti-Human/Mouse/Rat Plexin A2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5486) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the 66-440 kDa separation system.
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Plexin A2
Plexin A2 (also OCT) is a 220-230 kDa member of the plexin-A subfamily, plexin family of proteins. It is found on cerebellar granule cells, sensory neurons, and cardiac neural crest cells. It participates in cell migration and axon guidance, and does so by serving as a receptor for Sema6A and B, and as a coreceptor with neuropilin-1 for Sema3A and C. Mature mouse Plexin A2 is an 1860 amino acid (aa) type I transmembrane glycoprotein. It contains a 1203 aa extracellular domain (ECD) and a 636 aa cytoplasmic region. The ECD contains one Sema domain (aa 35-508), three PSI domains (aa 510-855) and four IPT regions (aa 858-1228). There is one alternate start site 10 aa upstream of the standard start site. Over aa 35-1237, mouse Plexin A2 shares 96% aa sequence identity with human Plexin A2.
Product Datasheets
Citations for Human/Mouse/Rat Plexin A2 Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Citations: Showing 1 - 5
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Semmaphorin 3?A causes immune suppression by inducing cytoskeletal paralysis in tumour-specific CD8+ T cells
Authors: Barnkob, MB;Michaels, YS;André, V;Macklin, PS;Gileadi, U;Valvo, S;Rei, M;Kulicke, C;Chen, JL;Jain, V;Woodcock, VK;Colin-York, H;Hadjinicolaou, AV;Kong, Y;Mayya, V;Mazet, JM;Mead, GJ;Bull, JA;Rijal, P;Pugh, CW;Townsend, AR;Gérard, A;Olsen, LR;Fritzsche, M;Fulga, TA;Dustin, ML;Jones, EY;Cerundolo, V;
Nature communications
Species: Mouse
Sample Types: Cell Lysates
Applications: Western Blot -
SEMA6A drives GnRH neuron-dependent puberty onset by tuning median eminence vascular permeability
Authors: Lettieri, A;Oleari, R;van den Munkhof, MH;van Battum, EY;Verhagen, MG;Tacconi, C;Spreafico, M;Paganoni, AJJ;Azzarelli, R;Andre', V;Amoruso, F;Palazzolo, L;Eberini, I;Dunkel, L;Howard, SR;Fantin, A;Pasterkamp, RJ;Cariboni, A;
Nature communications
Species: Mouse, Transgenic Mouse
Sample Types: Whole Cells, Whole Tissue
Applications: Immunohistochemistry, Immunocytochemistry -
Semaphorin3A/PlexinA3 association with the Scribble scaffold for cGMP increase is required for apical dendrite development
Authors: J Szczurkows, A Guo, J Martin, SI Lee, E Martinez, CT Chien, TA Khan, R Singh, D Dadson, TS Tran, S Pautot, M Shelly
Cell Reports, 2022-03-15;38(11):110483.
Species: Mouse, Rat
Sample Types: Whole Cells
Applications: ICC, IHC -
Sympathetic nerve repulsion inhibited by designer molecules in vitro and role in experimental arthritis
Authors: Rainer H Straub
J. Med. Microbiol., 2016-11-14;0(0):.
Species: Mouse
Sample Types: Whole Cells
Applications: Neutralization -
Neural crest-derived SEMA3C activates endothelial NRP1 for cardiac outflow tract septation.
Authors: Plein A, Calmont A, Fantin A, Denti L, Anderson N, Scambler P, Ruhrberg C
J Clin Invest, 2015-06-08;125(7):2661-76.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC
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