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cyclin B Antibody (D-1): sc-166210

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Datasheets
  • cyclin B Antibody (D-1) is a mouse monoclonal IgG1 κ cyclin B antibody, cited in 19 publications, provided at 200 µg/ml
  • raised against amino acids 1-300 of cyclin B of Drosophila melanogaster origin
  • cyclin B Antibody (D-1) is recommended for detection of cyclin B of d origin by WB, IP, IF and ELISA
  • Anti-cyclin B Antibody (D-1) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
  • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
  • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
  • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of cyclin B (D-1): sc-166210.
  • m-IgG Fc BP-HRP and m-IgG1 BP-HRP are the preferred secondary detection reagents for cyclin B Antibody (D-1) for WB applications. These reagents are now offered in bundles with cyclin B Antibody (D-1) (see ordering information below).

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Cyclin B Antibody (D-1) is a mouse monoclonal IgG1 kappa light chain antibody that detects cyclin B protein of Drosophila melanogaster origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). Anti-cyclin B antibody (D-1) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates, providing versatility for different experimental needs. Cyclin B (D-1) antibody recognizes a protein that plays a crucial role in cell cycle regulation, particularly in the transition from G2 phase to mitosis, by activating cyclin-dependent kinases (Cdks). This activation is essential for proper progression of the cell cycle, ensuring cells divide at the right time and under the right conditions. Precise regulation of cyclin B levels is vital, as dysregulation can lead to uncontrolled cell proliferation, a hallmark of cancer. In Drosophila, cyclin B expression peaks during metaphase, highlighting its importance in orchestrating mitosis events. Research on cyclin B in Drosophila melanogaster provides insights into fundamental cellular processes and serves as a model for understanding similar mechanisms in higher eukaryotes, making cyclin B (D-1) monoclonal antibody valuable for developmental biology and cancer research.

For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.

Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA

LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences

cyclin B Antibody (D-1) References:

  1. Cyclins and cell cycle checkpoints.  |  Johnson, DG. and Walker, CL. 1999. Annu Rev Pharmacol Toxicol. 39: 295-312. PMID: 10331086
  2. The genome sequence of Drosophila melanogaster.  |  Adams, MD., et al. 2000. Science. 287: 2185-95. PMID: 10731132
  3. Antizyme is a target of sex-lethal in the Drosophila germline and appears to act downstream of hedgehog to regulate sex-lethal and cyclin B.  |  Vied, C., et al. 2003. Dev Biol. 253: 214-29. PMID: 12645926
  4. 3' non-translated sequences in Drosophila cyclin B transcripts direct posterior pole accumulation late in oogenesis and peri-nuclear association in syncytial embryos.  |  Dalby, B. and Glover, DM. 1992. Development. 115: 989-97. PMID: 1451670
  5. Both cyclin B levels and DNA-replication checkpoint control the early embryonic mitoses in Drosophila.  |  Ji, JY., et al. 2004. Development. 131: 401-11. PMID: 14681192
  6. Expression of N-terminally truncated cyclin B in the Drosophila larval brain leads to mitotic delay at late anaphase.  |  Rimmington, G., et al. 1994. J Cell Sci. 107 (Pt 10): 2729-38. PMID: 7876341
  7. Cell cycle control of DNA replication.  |  Fotedar, R. and Fotedar, A. 1995. Prog Cell Cycle Res. 1: 73-89. PMID: 9552354

Ordering Information

Product NameCatalog #UNITPriceQtyFAVORITES

cyclin B Antibody (D-1)

sc-166210
200 µg/ml
$316.00

cyclin B Antibody (D-1): m-IgG Fc BP-HRP Bundle

sc-527144
200 µg Ab; 10 µg BP
$354.00

cyclin B Antibody (D-1): m-IgG1 BP-HRP Bundle

sc-532517
200 µg Ab; 20 µg BP
$354.00

cyclin B Antibody (D-1) AC

sc-166210 AC
500 µg/ml, 25% agarose
$416.00

cyclin B Antibody (D-1) HRP

sc-166210 HRP
200 µg/ml
$316.00

cyclin B Antibody (D-1) FITC

sc-166210 FITC
200 µg/ml
$330.00

cyclin B Antibody (D-1) PE

sc-166210 PE
200 µg/ml
$343.00

cyclin B Antibody (D-1) Alexa Fluor® 488

sc-166210 AF488
200 µg/ml
$357.00

cyclin B Antibody (D-1) Alexa Fluor® 546

sc-166210 AF546
200 µg/ml
$357.00

cyclin B Antibody (D-1) Alexa Fluor® 594

sc-166210 AF594
200 µg/ml
$357.00

cyclin B Antibody (D-1) Alexa Fluor® 647

sc-166210 AF647
200 µg/ml
$357.00

cyclin B Antibody (D-1) Alexa Fluor® 680

sc-166210 AF680
200 µg/ml
$357.00

cyclin B Antibody (D-1) Alexa Fluor® 790

sc-166210 AF790
200 µg/ml
$357.00

What blocking solution should I use with cyclin B (D-1): sc-166210 monoclonal antibody for Western blot?

Asked by: jerojero
Thank you for your question. We recommend blocking with Blotto (either TBS Blotto A: sc-2333 or TBS Blotto B: sc-2335). Our Western blot protocol can be found here: https://www.scbt.com/scbt/resources/protocols/western-immuno-blotting
Answered by: Technical Support
Date published: 2017-04-13
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Rated 5 out of 5 by from Very specific antibodyThis antibody works well with my project! No non-specific bands.
Date published: 2020-02-24
Rated 4 out of 5 by from Good featuresGood quality antibody for western blot detection of cyclin B in cell lysates with visible difference between proliferating and growth arrested cells
Date published: 2018-03-12
Rated 3 out of 5 by from an OK antibody for WBThis antibody generates a signal at the predicted position on the WB (1:500 dilution, 40ug HeLa extract), albeit the intensity is somewhat weak
Date published: 2017-09-18
Rated 4 out of 5 by from not suitable for turbotthere is a very light band when i exposed for 25min
Date published: 2017-07-10
Rated 1 out of 5 by from Didnot work for WBNo band was observed in MCF-7 cell lysates. Used the ab at 1:1000 dilution.
Date published: 2017-07-03
Rated 5 out of 5 by from Great featureThis antibody has been tested by WB for HepG2 cells. I used the product and diluted in TBST(1:200),4℃ overnight.
Date published: 2017-06-02
Rated 5 out of 5 by from show good resultIn my Western blot analysis,this cyclinB antibody show good performance.The band is very strong.
Date published: 2016-12-05
Rated 5 out of 5 by from It worked very nice on WB with Drosophila tissuesIt worked very nice on WB with Drosophila tissues.
Date published: 2015-05-19
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cyclin B Antibody (D-1) is rated 4.1 out of 5 by 9.
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