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MAD1 Antibody (117-470) is a mouse monoclonal IgG1 antibody that detects MAD1 in mouse, rat, and human samples through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). MAD1 plays a critical role in cell cycle regulation by ensuring proper chromosome alignment at the mitotic spindle assembly checkpoint, which is essential for preventing aneuploidy and maintaining genomic stability. MAD1 is primarily localized in the nucleus, where MAD1 is involved in anchoring MAD2L1 to the nuclear periphery, but during mitosis, MAD1 dynamically relocates to the centrosome, spindle midzone, and midbody, highlighting MAD1′s importance in the spatial regulation of mitotic processes. MAD1′s ability to form homo- and heterodimers with MAD2 is crucial for the formation of the tetrameric MAD1L1-MAD2L1 core complex, which is vital for the checkpoint function. Additionally, MAD1 is subject to hyperphosphorylation in response to spindle damage, a modification that enhances MAD1′s activity and underscores MAD1′s role in the cellular response to mitotic stress. Defects in the gene encoding MAD1, known as MAD1L1, have been implicated in the development and progression of various cancers, making MAD1 a significant target for research in cancer biology and therapeutic interventions.
Ordering Information
Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
MAD1 Antibody (117-470) | sc-65493 | 200 µg/ml | $316.00 |