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MAD1 Antibody (117-470): sc-65493

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Datasheets
  • MAD1 Antibody (117-470) is a mouse monoclonal IgG1 provided at 200 µg/ml
  • raised against full length MAD1 of human origin
  • recommended for detection of MAD1 of mouse, rat and human origin by WB, IP, IF and ELISA
  • At present, we have not yet completed the identification of the preferred secondary detection reagent(s) for MAD1 Antibody (117-470). This work is in progress.

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    MAD1 Antibody (117-470) is a mouse monoclonal IgG1 antibody that detects MAD1 in mouse, rat, and human samples through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). MAD1 plays a critical role in cell cycle regulation by ensuring proper chromosome alignment at the mitotic spindle assembly checkpoint, which is essential for preventing aneuploidy and maintaining genomic stability. MAD1 is primarily localized in the nucleus, where MAD1 is involved in anchoring MAD2L1 to the nuclear periphery, but during mitosis, MAD1 dynamically relocates to the centrosome, spindle midzone, and midbody, highlighting MAD1′s importance in the spatial regulation of mitotic processes. MAD1′s ability to form homo- and heterodimers with MAD2 is crucial for the formation of the tetrameric MAD1L1-MAD2L1 core complex, which is vital for the checkpoint function. Additionally, MAD1 is subject to hyperphosphorylation in response to spindle damage, a modification that enhances MAD1′s activity and underscores MAD1′s role in the cellular response to mitotic stress. Defects in the gene encoding MAD1, known as MAD1L1, have been implicated in the development and progression of various cancers, making MAD1 a significant target for research in cancer biology and therapeutic interventions.

    For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.

    Ordering Information

    Product NameCatalog #UNITPriceQtyFAVORITES

    MAD1 Antibody (117-470)

    sc-65493
    200 µg/ml
    $316.00

    Are there any issues with using sc-65493: MAD1 (117-470) mouse monoclonal primary antibody on mouse samples?

    Asked by: Dr Ninau Qelp
    Thank you for your inquiry. The use of mouse monoclonal antibodies with mouse samples is very common and typically poses no problem. To eliminate any potential cross-reactivity of an anti-mouse secondary detection reagent,we recommend using a directly conjugated primary antibody. Please contact our special orders department for pricing and availability of sc-65493 conjugates.
    Answered by: Technical Support
    Date published: 2017-03-07
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    Rated 4 out of 5 by from Strong band in WB in human transfected 293TStrong band in WB in human transfected 293T and HeLa whole cell lysates. -SCBT QC
    Date published: 2015-06-19
    Rated 4 out of 5 by from Good specificity in WB in HeLa and AGood specificity in WB in HeLa and A-431 whole cell lysates. -SCBT QC
    Date published: 2013-02-16
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    MAD1 Antibody (117-470) is rated 4.0 out of 5 by 2.
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