Render Target: STATIC
Render Timestamp: 2025-01-18T14:59:59.422Z
Commit: da7e4f2f0d1aed1f1f8e20e4e2ecab8f33cbd595
XML generation date: 2024-09-20 06:21:12.900
Product last modified at: 2024-09-20T07:11:00.588Z
Cell Signaling Technology Logo

Basket Updated

0

Items added

1% for the planet logo
PDP - Template Name: ELISA Antibody Pair
PDP - Template ID: *******c8d7b7a

PathScan® Total p21 Waf1/Cip1 Sandwich ELISA Antibody Pair #7856

Filter:
  • ELISA
PathScan® Total p21 Waf1/Cip1 Sandwich ELISA Antibody Pair: Image 1
The relationship between lysate protein concentration from 293 cells and the absorbance at 450 nm using PathScan® Total p21 Waf1/Cip1 Sandwich ELISA Antibody Pair is shown.
This product is discontinued

Inquiry Info. # 7856

Please see our recommended alternatives.

Supporting Data

REACTIVITY H
Application Key:
  • ELISA-ELISA 
Species Cross-Reactivity Key:
  • H-Human 

Product Information

Storage

Capture and detection antibodies are stored at 4°C. HRP-linked secondary reagent is stored at -20°C.

Protocol

Product Description

CST's PathScan® Total p21 Waf1/Cip1 Sandwich ELISA Antibody Pair is offered as an economical alternative to our PathScan®Total p21 Waf1/Cip1 Sandwich ELISA Kit #7167. Capture and detection antibodies (100X stocks) and HRP-conjugated secondary antibody (1000X stock) are supplied. Sufficient reagents are supplied for 4 x 96 well ELISAs. The p21 Waf1/Cip1 mouse capture antibody is coated on a 96 well microplate in PBS overnight. After blocking, cell lysates are added followed by a p21 Waf1/Cip1 rabbit detection antibody and anti-rabbit IgG, HRP conjugated antibody. HRP substrate, TMB, is added for color development. The magnitude of the absorbance for this developed color is proportional to the quantity of total p21 Waf1/Cip1 protein.
Antibodies in kit are custom formulations specific to kit.

Specificity / Sensitivity

For Antibody Pair specificity and sensitivity, please refer to the corresponding PathScan® Sandwich ELISA Kit. Note: This antibody pair detects proteins from the indicated species, as determined through in-house testing, but may also detect homologous proteins from other species.

Species Reactivity:

Human

Background

The tumor suppressor protein p21 Waf1/Cip1 acts as an inhibitor of cell cycle progression. It functions in stoichiometric relationships forming heterotrimeric complexes with cyclins and cyclin-dependent kinases. In association with CDK2 complexes, it serves to inhibit kinase activity and block progression through G1/S (1). However, p21 may also enhance assembly and activity in complexes of CDK4 or CDK6 and cyclin D (2). The carboxy-terminal region of p21 is sufficient to bind and inhibit PCNA, a subunit of DNA polymerase, and may coordinate DNA replication with cell cycle progression (3). Upon UV damage or during cell cycle stages when cdc2/cyclin B or CDK2/cyclin A are active, p53 is phosphorylated and upregulates p21 transcription via a p53-responsive element (4). Protein levels of p21 are downregulated through ubiquitination and proteasomal degradation (5).

Pathways

Explore pathways related to this product.


For Research Use Only. Not For Use In Diagnostic Procedures.
Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.
PathScan is a registered trademark of Cell Signaling Technology, Inc.
All other trademarks are the property of their respective owners. Visit our Trademark Information page.