SpecificityThis assay recognizes recombinant and natural mouse N-alpha-acetyltransferase 20. No significant cross-reactivity or interference was observed.
Intended UseMouse Naa20 ELISA Kit allows for the in vitro quantitative determination of Naa20 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Description specificalPrinciple of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to N-alpha-acetyltransferase 20. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for N-alpha-acetyltransferase 20 and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain N-alpha-acetyltransferase 20, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of N-alpha-acetyltransferase 20 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Mouse N-alpha-acetyltransferase 20 Protein information
Uniprot ID
NAA20_MOUSE
Uniprot AC
P61600; Q14B28; Q4VAC3; Q9D7H8; Q9H0Y4; Q9NQH6; Q9Y6D2;
UniGene
Mm.151168; Mm.442235;
GeneID
67877
KEGG
mmu:67877;
Mouse N-alpha-acetyltransferase 20 Protein SEQUENCE