Human NUP133 ELISA Kit

Alternative name
Human 133 kDa nucleoporin ELISA Kit;Human nucleoporin Nup133 ELISA Kit;Human hNUP133 ELISA Kit;Human nucleoporin 133 ELISA Kit;Human nuclear pore complex protein Nup133 ELISA Kit;Human nucleoporin 133kD ELISA Kit;Human nucleoporin 133kDa ELISA Kit;
Catalog
E027085
species
Human
GeneNUP133
Standard Curve
Other Species Mouse Nup133 ELISA Kit
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
Intended UseHuman NUP133 ELISA Kit allows for the in vitro quantitative determination of NUP133 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Categories/FamilySignal Transduction
Product Description
specificalFor Samples: Cell culture fluid & body fluid & tissue homogenate Serum or blood plasma Intended Uses: This NUP133 ELISA kit is intended for laboratory research use only and not for use in diagnostic or therapeutic procedures. The stop solution changes the color from blue to yellow and the intensity of the color is measured at 450 nm using a spectrophotometer. In order to measure the concentration of NUP133 in the sample, this NUP133 ELISA Kit includes a set of calibration standards. The calibration standards are assayed at the same time as the samples and allow the operator to produce a standard curve of Optical Density versus NUP133 concentration. The concentration of in the samples is then determined by comparing the O.D. of the samples to the standard curve. Principle of the Assay: The coated well immunoenzymatic assay for the quantitative measurement of NUP133 utilizes a multiclonal anti-NUP133 antibody and an NUP133-HRP conjugate. The assay sample and buffer are incubated together with NUP133-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the NUP133 concentration since NUP133 from samples and NUP133-HRP conjugate compete for the anti-NUP133 antibody binding site. Since the number of sites is limited, as more sites are occupied by NUP133 from the sample, fewer sites are left to bind NUP133-HRP conjugate. Standards of known NUP133 concentrations are run concurrently with the samples being assayed and a standard curve is plotted relating the intensity of the color (Optical Density) to the concentration of NUP133. The NUP133 concentration in each sample is interpolated from this standard curve.
Human Nuclear pore complex protein Nup133 Protein information
Uniprot ID NU133_HUMAN
Uniprot AC Q8WUM0; B2RAZ8; Q5T8N0; Q9H9W2; Q9NV71; Q9NVC4;
UniGene Hs.12457;
GeneID 55746
KEGG hsa:55746;
Human Nuclear pore complex protein Nup133 Protein SEQUENCE
SEQUENCE 1156 AA; 128979 MW; 78B733E353824577 CRC64;
MFPAAPSPRT PGTGSRRGPL AGLGPGSTPR TASRKGLPLG SAVSSPVLFS
PVGRRSSLSS RGTPTRMFPH HSITESVNYD VKTFGSSLPV KVMEALTLAE
VDDQLTINID EGGWACLVCK EKLIIWKIAL SPITKLSVCK ELQLPPSDFH
WSADLVALSY SSPSGEAHST QAVAVMVATR EGSIRYWPSL AGEDTYTEAF
VDSGGDKTYS FLTAVQGGSF ILSSSGSQLI RLIPESSGKI HQHILPQGQG
MLSGIGRKVS SLFGILSPSS DLTLSSVLWD RERSSFYSLT SSNISKWELD
DSSEKHAYSW DINRALKENI TDAIWGSESN YEAIKEGVNI RYLDLKQNCD
GLVILAAAWH SADNPCLIYY SLITIEDNGC QMSDAVTVEV TQYNPPFQSE
DLILCQLTVP NFSNQTAYLY NESAVYVCST GTGKFSLPQE KIVFNAQGDS
VLGAGACGGV PIIFSRNSGL VSITSRENVS ILAEDLEGSL ASSVAGPNSE
SMIFETTTKN ETIAQEDKIK LLKAAFLQYC RKDLGHAQMV VDELFSSHSD
LDSDSELDRA VTQISVDLMD DYPASDPRWA ESVPEEAPGF SNTSLIILHQ
LEDKMKAHSF LMDFIHQVGL FGRLGSFPVR GTPMATRLLL CEHAEKLSAA
IVLKNHHSRL SDLVNTAILI ALNKREYEIP SNLTPADVFF REVSQVDTIC
ECLLEHEEQV LRDAPMDSIE WAEVVINVNN ILKDMLQAAS HYRQNRNSLY
RREESLEKEP EYVPWTATSG PGGIRTVIIR QHEIVLKVAY PQADSNLRNI
VTEQLVALID CFLDGYVSQL KSVDKSSNRE RYDNLEMEYL QKRSDLLSPL
LSLGQYLWAA SLAEKYCDFD ILVQMCEQTD NQSRLQRYMT QFADQNFSDF
LFRWYLEKGK RGKLLSQPIS QHGQLANFLQ AHEHLSWLHE INSQELEKAH
ATLLGLANME TRYFAKKKTL LGLSKLAALA SDFSEDMLQE KIEEMAEQER
FLLHQETLPE QLLAEKQLNL SAMPVLTAPQ LIGLYICEEN RRANEYDFKK
ALDLLEYIDE EEDININDLK LEILCKALQR DNWSSSDGKD DPIEVSKDSI
FVKILQKLLK DGIQLSEYLP EVKDLLQADQ LGSLKSNPYF EFVLKANYEY
YVQGQI
UCSC uc001htn.4; human.;

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Human NUP133 ELISA Kit

Human 133 kDa nucleoporin ELISA Kit;Human nucleoporin Nup133 ELISA Kit;Human hNUP133 ELISA Kit;Human nucleoporin 133 ELISA Kit;Human nuclear pore complex protein Nup133 ELISA Kit;Human nucleoporin 133kD ELISA Kit;Human nucleoporin 133kDa ELISA Kit;