Human RSPO3 ELISA Kit

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  • Alternative name

    Human protein with TSP type-1 repeat ELISA Kit;Human hPWTSR ELISA Kit;Human roof plate-specific spondin-3 ELISA Kit;Human hRspo3 ELISA Kit;Human thrombospondin type-1 domain-containing protein 2 ELISA Kit;Human PWTSR ELISA Kit;Human THSD2 ELISA Kit;Human CRISTIN1 ELISA Kit;Human R-spondin 3 ELISA Kit;Human R-spondin-3 ELISA Kit;Human R-spondin 3 homolog ELISA Kit;Human thrombospondin, type I, domain containing 2 ELISA Kit;

  • Catalog
    E032616
  • species
    Human
  • GeneRSPO3
  • Standard CurveHuman RSPO3 ELISA Kit
  • Other Species Mouse Rspo3 ELISA KitBovine RSPO3 ELISA Kit
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of R-Spondin 3 (RSPO3). No significant cross-reactivity or interference between R-Spondin 3 (RSPO3) and analogues was observed.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • SensitivityTypically less than 0.118ng/mL.
  • Intended UseHuman RSPO3 ELISA Kit allows for the in vitro quantitative determination of RSPO3 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Description
    specifical
    Principle of the Assay: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to R-Spondin 3 (RSPO3). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to R-Spondin 3 (RSPO3). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain R-Spondin 3 (RSPO3), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of R-Spondin 3 (RSPO3) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
  • Human R-spondin-3 Protein information
  • Uniprot ID RSPO3_HUMAN
  • Uniprot AC Q9BXY4; B2RC27; Q5VTV4; Q96K87;
  • UniGene Hs.135254;
  • GeneID 84870
  • KEGG hsa:84870;
  • Human R-spondin-3 Protein SEQUENCE
  • SEQUENCE 272 AA; 30929 MW; CACAEC6B7E781189 CRC64;

    MHLRLISWLF IILNFMEYIG SQNASRGRRQ RRMHPNVSQG CQGGCATCSD

    YNGCLSCKPR LFFALERIGM KQIGVCLSSC PSGYYGTRYP DINKCTKCKA

    DCDTCFNKNF CTKCKSGFYL HLGKCLDNCP EGLEANNHTM ECVSIVHCEV

    SEWNPWSPCT KKGKTCGFKR GTETRVREII QHPSAKGNLC PPTNETRKCT

    VQRKKCQKGE RGKKGRERKR KKPNKGESKE AIPDSKSLES SKEIPEQREN

    KQQQKKRKVQ DKQKSVSVST VH

  • UCSC uc003qar.5; human. [Q9BXY4-1];


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