Render Target: STATIC
Render Timestamp: 2025-01-21T07:52:08.043Z
Commit: da7e4f2f0d1aed1f1f8e20e4e2ecab8f33cbd595
XML generation date: 2024-09-20 06:15:26.516
Product last modified at: 2025-01-01T09:05:12.799Z
Cell Signaling Technology Logo

Basket Updated

0

Items added

1% for the planet logo
PDP - Template Name: Chemical Modulators
PDP - Template ID: *******c501c72

Actinomycin D #15021

Actinomycin D: Image 1
Western blot analysis of extracts from MCF7 cells, untreated (-) or treated with Actinomycin D (1 μM) for the indicated times, using Phospho-Histone H2A.X (Ser139) (20E3) Rabbit mAb #9718 (upper) and Histone H2A.X (D17A3) XP® Rabbit mAb #7631 (lower).

To Purchase # 15021

Cat. # Size Qty. Price Ships
15021S 5 mg
$131 Jan 22
  • Related Products

Product Information

Product Usage Information

Actinoymcin D is supplied as a lyophilized powder. For a 10 mM stock, reconstitute the 5 mg in 398.28 μl DMSO. Working concentrations and length of treatment can vary depending on the desired effect, but it is typically used at 10-1,000 nM for 6-48 hr.

Storage

Store lyophilized or in solution at -20ºC, desiccated. Protect from light. In lyophilized form, the chemical is stable for 24 months. Once in solution, use within 1 week to prevent loss of potency. Aliquot to avoid multiple freeze/thaw cycles.

Product Description

Molecular Weight 1255.4 g/mol
Purity >98%
Molecular Formula C62H86N12O16
CAS 50-76-0
Solubility Soluble in DMSO at 50mg/ml.

Background

Actinomycin D is an effective anti-tumor agent and the most widely studied member of the actinomycin group of antibiotics (1). The compound, isolated from soil bacteria of the Streptomyces genus, is comprised of two cyclic pentapeptides bound by a phenoxazone group (2,3). Actinomycin D inhibits mRNA transcription in mammalian cells. Actinomycin D intercalates DNA and stabilizes topoisomerase I-DNA covalent complexes, blocking RNA chain elongation by RNA polymerase and consequently inhibiting protein synthesis (1,4,5). This mechanism of action induces p53-mediated cell cycle arrest in numerous cancer cell lines (6,7) and at high concentrations can induce apoptosis (8). Additional research studies show that Akt mediates actinomycin D-induced p53 expression (9).
For Research Use Only. Not For Use In Diagnostic Procedures.
Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.
XP is a registered trademark of Cell Signaling Technology, Inc.
All other trademarks are the property of their respective owners. Visit our Trademark Information page.