Home » Nurim » Nurim Lysates » Nurim Overexpression Lysate

Nurim Overexpression Lysate

 Submit a Review

Images

 
•
(
Enlarge)
Western Blot: Nurim Overexpression Lysate (Adult Normal) [NBP2-10376] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for Nurim.
Western Blot: Nurim Overexpression Lysate (Adult Normal) [NBP2-10376] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for Nurim.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB

Order Details

  • Loading...
Novus Biologicals offers 100% Guarantee
Datasheet
Reviews & Publications
Protocols & FAQs
Support & Research

Nurim Overexpression Lysate Summary

Description

Nurim Transient Overexpression Lysate


Expression Host: HEK293T

Plasmid: RC208090

Accession#: NM_007243

Protein Tag: C-MYC/DDK

You will receive 1 vial of lysate (100ug), 1 vial of empty vector negative control (100ug), and 1 vial of 2xSDS sample buffer (250ul). Each vial of cell lysate contains 100ug of total protein (at 1 mg/ml). The 2xSDS Sample Buffer consists of 4% SDS, 125mM Tris-HCl pH6.8, 10% Glycerol, 0.002% Bromophenol blue, 100mM DTT.
Gene
NRM

Applications/Dilutions

Dilutions
  • Western Blot
Application Notes
This product is intended for use as a positive control in Western Blot. Overexpression of the target protein was confirmed using an antibody to DDK (FLAG) epitope tag (NBP1-71705) present on the protein construct.

Each vial of cell lysate contains 100ug of total protein which should be sufficient for 20-50 reactions. Depending on over-expression level, antibody affinity and detection system, some lysates can go as low as 0.1 ug per load. We recommend starting with 5ug of cell lysate. Add an equal amount of cell lysate and 2X SDS Sample buffer and boil the SDS samples for 10 minutes before loading.
Theoretical MW
29.2 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at -80C. Avoid freeze-thaw cycles.
Buffer
RIPA buffer

Lysate Details for Array

Type
Overexpression

Notes

HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.

Alternate Names for Nurim Overexpression Lysate

  • NRM29Nuclear envelope membrane protein
  • Nuclear rim protein
  • nurim (nuclear envelope membrane protein)
  • nurim

Background

Nurim is an inner nuclear membrane (INM) protein that is tightly associated with the inner nuclear membrane. It was first isolated in a visual screen for nuclear envelope-localizing proteins. In subcellular fractionation, nurim remained extremely tightly bound to nuclear fractions. Unlike the known nuclear envelope (NE) membrane proteins, it is neither associated with nuclear pores, nor targeted like lamin-associated membrane proteins suggesting its localization to NE by a distinct mechanism.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

Customers Who Viewed This Item Also Viewed...

MID1 Antibody -
BSA Free
NBP1-26612
Western Blot MID1 Antibody - BSA Free
(3 Publications)
Species: Hu
Applications: IP (-), WB
PAG1 Antibody
(504004) [Unconjugate
MAB5285
Simple Western PAG1 Antibody (504004) [Unconjugated]
••
(1 Publication)
Species: Hu
Applications: Simple Western, WB

Customers Who Bought This Also Bought

Western Blot: c-Fos Antibody (2H2) [NBP2-50037] - Western blot shows lysates of HeLa human cervical epithelial carcinoma parental cell line and c-Fox knockout (KO) HeLa cell line. PVDF membrane was probed with 1:1000 of Mouse Anti-Human c-Fox Monoclonal Antibody (Catalog # NBP2-50037) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog #HAF018). Specific band was detected for c-Fox at approximately 52 kDa (as indicated) in the parental HeLa cell line, but is not detectable in the knockout HeLa cell line. This experiment was conducted under reducing conditions.
c-Fos Antibody (2H2) - BSA Free
NBP2-50037

Contact Information

Product PDFs

  SDS
  PDF Datasheet
  Request CofA

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our Nurim Overexpression Lysate and receive a gift card or discount.


Bioinformatics

Gene Symbol NRM