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BTN3A3 Recombinant Protein Antigen

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Reactivity HuSpecies Glossary
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BTN3A3 Recombinant Protein Antigen Summary

Description
A recombinant protein antigen with a N-terminal His6-ABP tag corresponding to human BTN3A3.

Source: E. coli

Amino Acid Sequence: SHIYTFPHASFSEPLYPVFRILTLEPTALTICPIPKEVESSPDPDLVPDHSLETPLTPGLANESGEPQAEVTSLLLPAHPGAEVSPSATTNQNHKLQARTEAL

Fusion Tag: N-terminal His6ABP (ABP = Albumin Binding Protein derived from Streptococcal Protein G)

This product is intended to be used as a blocking antigen for antibody competition assays. Any other use of this antigen is done at the risk of the user. The use of this product for commercial production is strictly prohibited. Please contact technical support if you have any questions.
Source
E. coli
Protein/Peptide Type
Recombinant Protein Antigen
Gene
BTN3A3
Purity
>80% by SDS-PAGE and Coomassie blue staining

Applications/Dilutions

Dilutions
  • Antibody Competition 10 - 100 molar excess
Application Notes
This recombinant antigen is only intended to be used as a blocking agent to confirm antibody specificity with the corresponding antibody, catalog number NBP1-88509.

It is purified by IMAC chromatography, and the expected concentration is greater than 0.5 mg/ml.

For current lot information, including availability, please contact our technical support team click nb-technical@bio-techne.com

Theoretical MW
29 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
PBS and 1M Urea, pH 7.4.
Preservative
No Preservative
Purity
>80% by SDS-PAGE and Coomassie blue staining

Alternate Names for BTN3A3 Recombinant Protein Antigen

  • BTF3
  • BTF3butyrophilin 3
  • BTN3.3
  • BTN3A3
  • butyrophilin subfamily 3 member A3
  • butyrophilin, subfamily 3, member A3

Background

BTN3A3 belongs to the BTN3 subfamily of the butyrophilin (BTN) family. BTN genes are MHC-associated and code for membrane proteins. BTN3A1, BTN3A2 and BTN3A3 exhibit very high homology, but only BTN3A1 and BTN3A3 have an intracellular B30.2 (SPRY) domain in addition to the two extracellular Ig domains. BTN3 (CD277) molecules are found on various immune cells.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

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Bioinformatics

Gene Symbol BTN3A3