This protein was produced in an in vitro wheat germ expression system that should preserve correct conformational folding that is necessary for biological function. While it is possible that this protein could display some level of activity, the functionality of this protein has not been explicitly measured or validated.
Source
Wheat germ
Protein/Peptide Type
Recombinant Protein
Gene
YBX1
Purity
>80% by SDS-PAGE and Coomassie blue staining
Applications/Dilutions
Dilutions
ELISA
Immunoaffinity Purification
Protein Array
Western Blot
Theoretical MW
62.3 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
50 mM Tris-HCI, 10 mM reduced Glutathione, pH 8.0.
Purity
>80% by SDS-PAGE and Coomassie blue staining
Notes
This product is produced by and distributed for Abnova, a company based in Taiwan.
Alternate Names for Recombinant Human YB1 Protein
BP-8
CBF-A
class II, Y box-binding protein I
CSDA2
CSDB
DBPB CCAAT-binding transcription factor I subunit A
EFI-A
Enhancer factor I subunit A
MDR-NF1
MGC104858
MGC110976
MGC117250
NSEP1 Y-box-binding protein 1
nuclease-sensitive element-binding protein 1
Y box binding protein 1
YB1 DNA-binding protein B
YB-1 Y-box transcription factor
YBX1
Background
The Y-box binding protein 1 (YB1) is a pluripotent DNA/RNA-binding factor which regulates gene expression through transcription and translation. YB1 has been shown to be a marker of tumor aggressiveness and belongs to the cold-shock domain family.
Limitations
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.
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Product General Protocols
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FAQs for YB1 Recombinant Protein (H00004904-P01). (Showing 1 - 1 of 1 FAQ).
I am ordering your product YB1 Recombinant Protein, H00004904-P01. I am going to use this product for checking its activity (ability to bind RNA) in translation assay experiments. I would like to know the following technical information about this product: 1) Is the recombinant protein is active? 2) Which condition is the protein is purified: native or denaturing? 3) Please provide a reference/s on the use of this product in case it is available. ..show answer..
The answers to your questions are as follows: 1) This recombinant protein has not been tested for any functionality; we therefore recommend that it is not used for experiments requiring activity. 2) The method of protein purification used is Glutathione Sepharose 4 Fast Flow; the tagged proteins are eluted under mild, non-denaturing conditions. 3) Here is a MCB reference for H00004904-P01 (where the manufacturer is shown as Abnova, the Taiwanese company we distribute this product for).
New Techniques Using Phosphoserine Antibodies Phosphoserine, the phosphorylated modification of the amino acid serine, is a central post-translational modification within a cell for many biological and biomedical processes. The phosphorylation of specifically four residue types - histidine, serin... Read full blog post.
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