SignalSilence® DNMT3L siRNA I (Mouse Specific) #12307
Inquiry Info. # 12307
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Supporting Data
REACTIVITY | M |
Species Cross-Reactivity Key:
- M-Mouse
Product Information
Product Usage Information
CST recommends transfection with 100 nM SignalSilence® DNMT3L siRNA I (Mouse Specific) 48 to 72 hours prior to cell lysis. For transfection procedure, follow protocol provided by the transfection reagent manufacturer. Please feel free to contact CST with any questions on use.
Each vial contains the equivalent of 100 transfections, which corresponds to a final siRNA concentration of 100 nM per transfection in a 24-well plate with a total volume of 300 μl per well.
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Product Description
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Background
DNMT3L is a catalytically inactive regulatory factor for the DNMT3A and DNMT3B de novo methyltransferases that is expressed at low levels in embryonic stem cells, testis, ovaries, and thymus (1,2). These de novo methyltransferases consist of a heterotetrameric complex containing two molecules of DNMT3L, and either two molecules of DNMT3A or DNMT3B (3). DNMT3L contains an amino-terminal ATRX-DNMT3-DNMT3L (ADD) domain and a carboxy-terminal methyltransferase-like domain (4-7). The methyltransferase-like domain binds to DNMT3A and DNMT3B to stimulate catalytic activity by increasing the binding of S-adenosylmethionine and DNA (4,5). The ADD domain recruits the methyltransferase complex to transcriptionally inactive regions of the genome by binding to unmethylated histone H3 Lys4 (6,7).
- Hermann, A. et al. (2004) Cell Mol Life Sci 61, 2571-87.
- Turek-Plewa, J. and Jagodziński, P.P. (2005) Cell Mol Biol Lett 10, 631-47.
- Jia, D. et al. (2007) Nature 449, 248-51.
- Holz-Schietinger, C. and Reich, N.O. (2010) J Biol Chem 285, 29091-100.
- Suetake, I. et al. (2004) J Biol Chem 279, 27816-23.
- Ooi, S.K. et al. (2007) Nature 448, 714-7.
- Otani, J. et al. (2009) EMBO Rep 10, 1235-41.
Limited Uses
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