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CD274/PD-L1 CRISPR Plasmids (h)

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Datasheets
  • Target species: human; for corresponding mouse product, see CD274/PD-L1 CRISPR Plasmids (m)
  • CRISPR/Cas9 KO Plasmids consists of CD274/PD-L1-specific 20 nt guide RNA sequences derived from the GeCKO (v2) library
  • For CRISPR gene knockout, gRNA sequences direct the Cas9 protein to induce a site-specific double strand break (DSB) in the genomic DNA
  • Target-specific CRISPR Plasmids for both gene knockout and activation are available. Please refer to the detailed product information in the tabs below
  • Gene knockdown or activation can be assayed using CD274/PD-L1 Antibody (1C10): sc-293425
  • All products are provided as transfection-ready, purified plasmid DNA, except the Lentiviral Activation Particles which have been prepackaged as Lentiviral Particles for use with hard-to-transfect cells

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Description

  • 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
  • CD274/PD-L1 CRISPR/Cas9 Knockout (KO) Plasmid (h2) consists of a pool of three plasmids each encoding the Cas9 nuclease and a CD274/PD-L1-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency
  • gRNA sequences are derived from the GeCKO (v2) library and direct the Cas9 protein to induce a site-specific double strand break (DSB) in the genomic DNA
  • For selection of cells containing a successful Cas9-induced DSB, co-transfection with CD274/PD-L1 HDR Plasmid (h2) (sc-401140-HDR-2) is recommended
  • Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: CD274/PD-L1 Antibody (1C10): sc-293425

See Protocol Online.

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Description

  • 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
  • CD274/PD-L1 HDR Plasmid (h2) is recommended for co-transfection with CD274/PD-L1 CRISPR/Cas9 KO Plasmid (h2)
  • CD274/PD-L1 HDR Plasmid (h2) consists of a pool of 2-3 plasmids, each containing a homology-directed DNA repair (HDR) template corresponding to the cut sites generated by the CD274/PD-L1 CRISPR/Cas9 KO Plasmid (h2)
  • Each HDR plasmid contains two 800 bp homology arms designed to specifically bind to the genomic DNA surrounding the corresponding Cas9-induced double strand DNA break site
  • Each HDR Plasmid inserts a puromycin resistance gene to enable selection of stable knockout (KO) cells and an RFP (Red Fluorescent Protein) gene to visually verify transfection
  • The puromycin resistance and RFP genes are flanked by two LoxP sites to allow for further processing by the Cre Vector sc-418923

See Protocol Online.

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Description

  • 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
  • CD274/PD-L1 Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
  • Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
  • One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
  • The paired gRNAs encoded by CD274/PD-L1 Double Nickase Plasmid (h2) differ from the paired gRNAs in CD274/PD-L1 Double Nickase Plasmid (h)
  • Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: CD274/PD-L1 Antibody (1C10): sc-293425

See Protocol Online.

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Description

  • 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
  • CD274/PD-L1 CRISPR Activation Plasmid (h) is a synergistic activation mediator (SAM) transcription activation system designed to specifically upregulate gene expression
  • CD274/PD-L1 CRISPR Activation Plasmid (h) consists of three plasmids at a 1:1:1 mass ratio: a plasmid encoding the deactivated Cas9 (dCas9) nuclease (D10A and N863A) fused to the transactivation domain VP64, and a blasticidin resistance gene; a plasmid encoding the MS2-p65-HSF1 fusion protein, and a hygromycin resistance gene; a plasmid encoding a target-specific 20 nt guide RNA fused to two MS2 RNA aptamers, and a puromycin resistance gene
  • The resulting SAM complex binds to a site-specific region approximately 200-250 nt upstream of the transcriptional start site and provides robust recruitment of transcription factors for highly efficient gene activation
  • Following transfection, gene activation efficiency can be assayed by WB, IF or IHC using antibody: CD274/PD-L1 Antibody (1C10): sc-293425

See Protocol Online.

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Description

  • 200 µl of transduction-ready, high-titer CRISPR/dCas9 Lentiviral Activation Particles
  • CD274/PD-L1 Lentiviral Activation Particles (h) is a synergistic activation mediator (SAM) transcription activation system designed to specifically and efficiently upregulate gene expression via lentiviral transduction of cells
  • CD274/PD-L1 Lentiviral Activation Particles (h) contain the following SAM Activation elements: a deactivated Cas9 (dCas9) nuclease (D10A and N863A) fused to the transactivation domain VP64, an MS2-p65-HSF1 fusion protein and a target-specific 20 nt. guide RNA. They also contain the blasticidin, hygromycin and puromycin resistance genes
  • Upon transduction, the SAM complex binds to a site-specific region approximately 200-250 nt. upstream of the transcriptional start site and provides robust recruitment of transcription factors for highly efficient gene activation
  • The guide RNA in the CD274/PD-L1 Lentiviral Activation Particles (h2) encodes a target-specific 20 nt guide RNA that differs from the guide RNA in CD274/PD-L1 Lentiviral Activation Particles (h)
  • Following transfection, gene activation efficiency can be assayed by WB, IF or IHC using antibody: CD274/PD-L1 Antibody (1C10): sc-293425

See Protocol Online.

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Ordering Information

Product NameCatalog #UNITPriceQtyFAVORITES

CD274/PD-L1 CRISPR/Cas9 KO Plasmid (h2)

sc-401140-KO-2
20 µg
$383.00

CD274/PD-L1 HDR Plasmid (h2)

sc-401140-HDR-2
20 µg
$429.00

CD274/PD-L1 Double Nickase Plasmid (h)

sc-401140-NIC
20 µg
$395.00

CD274/PD-L1 Double Nickase Plasmid (h2)

sc-401140-NIC-2
20 µg
$395.00

CD274/PD-L1 CRISPR Activation Plasmid (h)

sc-401140-ACT
20 µg
$383.00

CD274/PD-L1 CRISPR Activation Plasmid (h2)

sc-401140-ACT-2
20 µg
$383.00

CD274/PD-L1 Lentiviral Activation Particles (h)

sc-401140-LAC
200 µl
$439.00

CD274/PD-L1 Lentiviral Activation Particles (h2)

sc-401140-LAC-2
200 µl
$439.00