Western blot analysis of extracts from HeLa cells, transfected with 100 nM SignalSilence ® Control siRNA (Unconjugated) #6568 (-), SignalSilence ® Glucocorticoid Receptor siRNA I #6508 (+), or SignalSilence ® Glucocorticoid Receptor siRNA II (+), using Glucocorticoid Receptor (D8H2) XP ® Rabbit mAb #3660 (upper) or GAPDH (D16H11) XP ® Rabbit mAb #5174 (lower). The Glucocorticoid Receptor (D8H2) XP ® Rabbit mAb confirms silencing of glucocorticoid receptor expression, while the GAPDH (D16H11) XP ® Rabbit mAb is used as a loading control.