Western Blot: Akt1 RNAi [H00000207-R02V] - Analysis of AKT1 over-expressed 293 cell line. Lane 2 cotransfected with AKT1 Validated Chimera RNAi Lane 1 non-transfected control. Blot probed with H00000207-M01. GAPDH ( 36.1 kDa ) used as loading control.