MISSION® esiRNA

Name	MISSION® esiRNA
Supplier	Sigma-Aldrich
Catalog	EHU106441
Category	siRNA
Prices	$140.00, $250.00
Sizes	20 µg, 50 µg
Applications	Gene Silencing
Gene	PTEN
Sequence	ACCGCCAAATTTAATTGCAGAGTTGCACAATATCCTTTTGAAGACCATAACCCACCACAGCTAGAACTTATCAAACCCTTTTGTGAAGATCTTGACCAATGGCTAAGTGAAGATGACAATCATGTTGCAGCAATTCACTGTAAAGCTGGAAAGGGACGAACTGGTGTAATGATATGTGCATATTTATTACATCGGGGCAAATTTTTAAAGGCACAAGAGGCCCTAGATTTCTATGGGGAAGTAAGGACCAGAGACAAAAAGGGAGTAACTATTCCCAGTCAGAGGCGCTATGTGTATTATTATAGCTACCTGTTAAAGAATCATCTGGATTATAGACCAGTGGCACTGTTGTTTCACAAGATGATGTTTGAAACTATTCCAATGTTCAGTGGCGGAACTTGCAATCCTCAGTTTGTGGTCTGCCAGCTAAAGGTGAAGATATATTCCTCCAATTCAGGACCCACACGACGGGAAGACAAGTTCATGTACTTTGAGTTCCCTCAGCCGTTACCT
Description	MISSION esiRNA are endo-ribonuclease prepared siRNA
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Product images

<B>Immunoblotting</B><BR/>PTEN: <B>Cat. No. EHU106441</B>: Western blot analysis of PTEN in HeLa cells.<BR/><B>Lanes</B><BR/>1. HeLa cells transfected with Renilla Luciferase esiRNA (RLUC, <B>Cat. No. EHURLUC</B>) as negative control.<BR/>2. HeLa cells transfected with PTEN esiRNA (<B>Cat. No. EHU106441</B>). Quantitative-Immunoblotting: Lysates were separated on SDS-Page and probed with mouse Monoclonal Anti-PTEN (1:200) Clone: PTN-18 (<B>Cat. No. P3487</B>) and goat anti GAPDH (1:7000) (Acris) antibodies. The signal intensities of indicated bands were quantified using an Odyssey infrared imaging system (Li-COR) and normalized to the GAPDH signal. At 72h post transfection, an 83% PTEN protein reduction was measured between the negative control RLUC and the sample treated with PTEN esiRNA.

Immunoblotting
PTEN: Cat. No. EHU106441: Western blot analysis of PTEN in HeLa cells.
Lanes
1. HeLa cells transfected with Renilla Luciferase esiRNA (RLUC, Cat. No. EHURLUC) as negative control.
2. HeLa cells transfected with PTEN esiRNA (Cat. No. EHU106441). Quantitative-Immunoblotting: Lysates were separated on SDS-Page and probed with mouse Monoclonal Anti-PTEN (1:200) Clone: PTN-18 (Cat. No. P3487) and goat anti GAPDH (1:7000) (Acris) antibodies. The signal intensities of indicated bands were quantified using an Odyssey infrared imaging system (Li-COR) and normalized to the GAPDH signal. At 72h post transfection, an 83% PTEN protein reduction was measured between the negative control RLUC and the sample treated with PTEN esiRNA.