Anti-DTX4 antibody - N-terminal (ab175837) at 1/1000 dilution + HL60 cell line lysate at 35 µg
Immunohistochemical analysis of formalin fixed, paraffin embedded Human brain tissue labeling DTX4 with ab175837 at 1/50 followed by peroxidase conjugation of the secondary antibody and DAB staining.
Flow Cytometrical analysis of HeLa cells labeling DTX4 with ab175837 at 1/10 (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.