Anti-Dynamin 2 antibody

• Supplier: Abcam
• Catalog: ab3457
• Prices: $401.00
• Sizes: 100 µl
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Applications: WB ICC/IF IP ICC/IF ICC/IF IHC-F IHC-P
• Species Reactivities: Mouse, Rat, Human, Primate, Bovine
• Antigen: Synthetic peptide corresponding to Human Dynamin 2 aa 760-779
• Description: Rabbit Polyclonal
• Gene: DNM2
• Conjugate: Unconjugated

Product images

Western blot detection of Dynamin 2 on mouse testes homogenate using ab3457.

ab3457 (1µg/ml) staining Dynamin 2 in human lung using an automated system (DAKO Autostainer Plus).Sections were rehydrated and antigen retrieved with the Dako 3 in 1 AR buffers EDTA pH 9.0 in a DAKO PT link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 mins. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 min and detected with Dako envision flex amplification kit for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that, for manual staining, optimization of primary antibody concentration and incubation time is recommended. Signal amplification may be required.

ICC/IF image of ab3457 stained HeLa cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab3457, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

ab3457 staining Dynamin 2 in rat testis tissue by Immunohistochemistry (Frozen sections). Samples were dual-labeled.Frozen testis sections (7 µm) were fixed with 4% paraformaldehyde (wt/vol) for 10 minutes and permeabilized in 0.1% Triton X-100 (vol/vol) for 4 minutes. Sections were blocked in 1% BSA (wt/vol) for 30 minutes, followed by an overnight incubation of ab3457 and an anti-Arp3 antibody, both at a 1/200 dilution (diluted in 1% BSA). Samples were then incubated for 30 minutes with an Alexa Fluor 488 (green) conjugated secondary, and an Alexa Fluor 555 (red) conjugated secondary, at a dilution of 1/100–1/250 containing 1% BSA. All incubations were performed at room temperature. Counterstained with DAPI (blue).

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