Anti-Dynamitin antibody
| Name | Anti-Dynamitin antibody |
|---|---|
| Supplier | Abcam |
| Catalog | ab56687 |
| Prices | $388.00 |
| Sizes | 100 µg |
| Host | Mouse |
| Clonality | Monoclonal |
| Isotype | IgG1 |
| Applications | WB FC |
| Species Reactivities | Mouse, Human |
| Antigen | Recombinant fragment: DADTQSKVHQ LYETIQRWSP IASTLPELVQ RLVTIKQLHE QAMQFGQLLT HLDTTQQMIA NSLKDNTTLL TQVQTTMREN LATVEGNFAS IDERMKKLGK , corresponding to amino acids 302-401 of Human Dynamitin Run BLAST with Run BLAST with |
| Description | Mouse Monoclonal |
| Gene | DCTN2 |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
Dynamitin antibody (ab56687) at 1ug/lane + MCF-7 cell lysate at 25ug/lane.
Anti-Dynamitin antibody (ab56687) at 1/500 dilution (in TBS-Tween + 5% milk for 1 hour at 4°C) + Whole cell lysate of HeLa cells at 20 µgSecondaryAn IRDye®800CW-conjugated Goat anti-mouse polyclonal at 1/10000 dilutionPerformed under reducing conditions.Observed band size : 50 kDa (why is the actual band size different from the predicted?)Additional bands at : 74 kDa. We are unsure as to the identity of these extra bands.Exposure time : 10 minutesThis image is courtesy of an anonymous AbreviewSee Abreview
ab55687 staining Dynamitin in HeLa cells by Immunocytochemistry/ Immunofluorescence. The cells were fixed in formaldehyde, permeabilised in 0.5% Triton X and then blocked using 3% BSA for 1 hour at 24°C. Samples were then incubated with the primary antibody at 1/100 for 1 hour at 24°C. The secondary antibody used was a chicken anti-mouse IgG conjugated to Alexa Fluor® 488 (green) used at a 1/1000 dilution.See Abreview
Total protein input, Hela cell lysate: 500µg.Matrix used for Immunoprecipitation step: Protein A Dynabeads.ab56687 used at a 1/1000 dilution, 20µl/mg lysate.LYS - Lysates BC - Beads + Lysates IP - Beads + Ab + LysatesSee Abreview
![Overlay histogram showing HeLa cells stained with ab56687 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab56687, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_2/10758_Dynamitin-Primary-antibodies-ab56687-12.jpg)
Overlay histogram showing HeLa cells stained with ab56687 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab56687, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.
IHC image of Dynamitin staining in Human breast fibroadenoma formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab56687, 10µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
Product References
Prenatal alcohol exposure modifies glucocorticoid receptor subcellular - Prenatal alcohol exposure modifies glucocorticoid receptor subcellular
Allan AM, Goggin SL, Caldwell KK. PLoS One. 2014 Apr 22;9(4):e96200.
Rotational motion during three-dimensional morphogenesis of mammary epithelial - Rotational motion during three-dimensional morphogenesis of mammary epithelial
Wang H, Lacoche S, Huang L, Xue B, Muthuswamy SK. Proc Natl Acad Sci U S A. 2013 Jan 2;110(1):163-8.