DCTN2

Gene Symbol DCTN2
Entrez Gene 10540
Alt Symbol DCTN50, DYNAMITIN, HEL-S-77, RBP50
Species Human
Gene Type protein-coding
Description dynactin 2 (p50)
Other Description 50 kD dynein-associated polypeptide|50 kDa dynein-associated polypeptide|dynactin complex 50 kD subunit|dynactin complex 50 kDa subunit|dynactin subunit 2|epididymis secretory protein Li 77|p50 dynamitin
Swissprots Q9BW17 Q86YN2 B2RBK5 Q13561
Accessions EAW97027 EAW97028 EAW97029 Q13561 AK055682 BAG51555 AK096242 BAG53236 AK098794 AK222693 BAD96413 AK292364 BAF85053 AK314705 BAG37252 AY189155 AAO34395 BC000718 AAH00718 BC009468 AAH09468 BC014083 AAH14083 DB027733 DB569305 FJ224315 ACI46007 HQ447201 ADQ31688 U50733 AAC50423 XM_011537761 XP_011536063 XR_944481 NM_001261412 NP_001248341 NM_001261413 NP_001248342 NM_006400 NP_006391
Function Modulates cytoplasmic dynein binding to an organelle, and plays a role in prometaphase chromosome alignment and spindle organization during mitosis. Involved in anchoring microtubules to centrosomes. May play a role in synapse formation during brain development.
Subcellular Location Cytoplasm, cytoskeleton, microtubule organizing center, centrosome {ECO:0000269|PubMed:8647893}. Membrane {ECO:0000269|PubMed:8647893}; Peripheral membrane protein {ECO:0000269|PubMed:8647893}.
Top Pathways Huntington's disease, Vasopressin-regulated water reabsorption

Dynactin 2 (G-4) - sc-393389 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
Dynactin 2 (G-4): sc-393389. Western blot analysis of Dynactin 2 expression in MCF7 (A) and MCP-5 (B) whole cell lysates.

Dynactin 2 (E-15) - sc-167697 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
Dynactin 2 (E-15): sc-167697. Western blot analysis of Dynactin 2 expression in K-562 (A), HL-60 (B) and AML-193 (C) whole cell lysates.

Anti-Dynamitin antibody - ab56687 from Abcam

Prices $388.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal
Dynamitin antibody (ab56687) at 1ug/lane + MCF-7 cell lysate at 25ug/lane.

Anti-Dynamitin antibody - ab185928 from Abcam

Prices $378.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Immunocytochemistry/Immunofluorescence analysis of U2OS cells using ab185928. Blue DAPI for nuclear staining.

Anti-Dynamitin antibody - ab124285 from Abcam

Prices $376.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
All lanes : Anti-Dynamitin antibody (ab124285) at 0.1 µg/mlLane 1 : Jurkat whole cell lysate at 50 µgLane 2 : Jurkat whole cell lysate at 15 µgLane 3 : 293T whole cell lysate at 50 µgLane 4 : HeLa whole cell lysate at 50 µgLane 5 : NIH3T3 whole cell lysate at 50 µgdeveloped using the ECL techniqueExposure time : 30 seconds

Anti-Dynamitin antibody - ab89118 from Abcam

Prices $376.00
Sizes 50 µg
Host Mouse
Clonality Polyclonal
Anti-Dynamitin antibody (ab89118) at 1 µg/ml + Human spleen tissue lysate at 50 µg

Anti-Dynamitin antibody - ab171398 from Abcam

Prices $376.00
Sizes 400 µl
Host Rabbit
Clonality Polyclonal
All lanes : Anti-Dynamitin antibody (ab171398) at 1/50 dilutionLane 1 : Lysate from non transfected 293 cellsLane 2 : Lysate from 293 cells transiently transfected with DynamitinLysates/proteins at 2 µg per lane.

Anti-Dynamitin antibody - ab53714 from Abcam

Prices $378.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
All lanes : Anti-Dynamitin antibody (ab53714) at 1/500 dilutionLane 1 : Extracts from A549 cellsLane 2 : Extracts from A549 cells, plus immunising peptide

Anti-Dynamitin antibody [EPR5094] - ab109752 from Abcam

Prices $379.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EPR5094
All lanes : Anti-Dynamitin antibody [EPR5094] (ab109752) at 1/10000 dilutionLane 1 : MCF7 cell lysateLane 2 : A375 cell lysateLane 3 : Saos2 cell lysateLane 4 : SH Y5Y cell lysateLysates/proteins at 10 µg per lane.

Anti-Dynamitin antibody [EPR5095] - ab133492 from Abcam

Prices $379.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EPR5095
All lanes : Anti-Dynamitin antibody [EPR5095] (ab133492) at 1/1000 dilutionLane 1 : MCF7 cell lysateLane 2 : A375 cell lysateLane 3 : Saos2 cell lysateLane 4 : SH SY5Y cell lysateLysates/proteins at 10 µg per lane.SecondaryHRP labelled goat anti-rabbit at 1/2000 dilution

Anti-Dynactin 2 / Dynamitin Antibody (aa277-401) - LS-C182838 from LifeSpan Bioscience

Prices $650.00
Sizes 100 µg
Host Sheep
Clonality Polyclonal

Anti-Dynactin 2 / Dynamitin Antibody (aa1-50) - LS-C290160 from LifeSpan Bioscience

Prices $375.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal

Anti-Dynactin 2 / Dynamitin Antibody (N-Terminus) - LS-C112194 from LifeSpan Bioscience

Prices $365.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal
DCTN2 antibody LS-C112194 Western blot of Jurkat lysate.

Anti-Dynactin 2 / Dynamitin Antibody (Leu371) - LS-C176158 from LifeSpan Bioscience

Prices $345.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
Western blot of p50 Dynamitin (L371) pAb in extracts from A549 cells.

Anti-Dynactin 2 / Dynamitin Antibody - LS-C193105 from LifeSpan Bioscience

Prices $385.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
Western blot.

Anti-Dynactin 2 / Dynamitin Antibody (aa341-390) - LS-C118546 from LifeSpan Bioscience

Prices $315.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
Western blot of extracts from A549 cells, using Dynamitin Antibody. The lane on the right is treated with the synthesized peptide.

Anti-Dynactin 2 / Dynamitin Antibody (aa351-401) - LS-C290161 from LifeSpan Bioscience

Prices $375.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal

Anti-Dynactin 2 / Dynamitin Antibody - LS-C307191 from LifeSpan Bioscience

Prices $455.00
Sizes 50 µg
Host Mouse
Clonality Polyclonal

Anti-Dynactin 2 / Dynamitin Antibody (aa302-401) - LS-C307192 from LifeSpan Bioscience

Prices $455.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal

Anti-Dynactin 2 / Dynamitin Antibody - LS-C310094 from LifeSpan Bioscience

Prices $475.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal

Anti-Dynactin 2 / Dynamitin Antibody (Internal, clone EPR5095) - LS-C138491 from LifeSpan Bioscience

Prices $475.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EPR5095
Immunofluorescent staining of SH-SY5Y cells using anti-DCTN2 antibody.

Anti-Dynactin 2 / Dynamitin Antibody (aa192-219) - LS-C167306 from LifeSpan Bioscience

Prices $295.00
Sizes 400 µl
Host Rabbit
Clonality Polyclonal
DCTN2 Antibody immunohistochemistry of formalin-fixed and paraffin-embedded human stomach tissue followed by peroxidase-conjugated secondary antibody and DAB staining.

Anti-Dynactin 2 / Dynamitin Antibody (Internal, clone EPR5094) - LS-C138345 from LifeSpan Bioscience

Prices $475.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EPR5094
Western blot analysis on (A) MCF-7, (B) A375, (C) Saos-2, and (D) SH-SY5Y cell lysates using anti-DCTN2 antibody.

Anti-Dynactin 2 / Dynamitin Antibody - LS-C331980 from LifeSpan Bioscience

Prices $255.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal
Immunohistochemistry of paraffin-embedded human kidney using DCTN2 antibody at dilution of 1:200 (x400 lens).

Dynactin Subunit 2/DCTN2/DCTN-50 Antibody - AF6850 from Novus Biologicals

Prices $99.00, $359.00
Sizes 25 µg, 100 µg
Host Sheep
Clonality Polyclonal
Western blot shows lysates of human placenta tissue and CTLL‑2 mouse cytotoxic T cell line. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human Dynactin Subunit 2/DCTN2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6850) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for Dynactin Subunit 2/DCTN2 at approximately 54 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
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