All lanes : Anti-E2F6 antibody [EPR11201] (ab155978) at 1/1000 dilutionLane 1 : Jurkat cell lysateLane 2 : K562 cell lysateLane 3 : HeLa cell lysateLane 4 : A549 cell lysateLane 5 : HepG2 cell lysateLysates/proteins at 10 µg per lane.SecondaryGoat anti-rabbit HRP at 1/2000 dilution
Immunofluorescent analysis of HeLa cells, labeling E2F6 with ab155978 at 1/100 dilution.
Flow cytometric analysis of permeabilized K562 cells labeling E2F6 (red), with ab155978 at 1/10 dilution, or a Rabbit IgG (negative) (green)