Anti-E2F6 antibody - ChIP Grade
| Name | Anti-E2F6 antibody - ChIP Grade |
|---|---|
| Supplier | Abcam |
| Catalog | ab53061 |
| Prices | $395.00 |
| Sizes | 100 µg |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Applications | IP ChIP WB ELISA |
| Species Reactivities | Mouse, Human |
| Antigen | A synthesized peptide derived from human E2F6 |
| Description | Rabbit Polyclonal |
| Gene | E2F6 |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
All lanes : Anti-E2F6 antibody - ChIP Grade (ab53061) at 1/500 dilutionLane 1 : Extracts of HepG2 cellsLane 2 : Extracts of HepG2 cells treated with peptide
All lanes : Anti-E2F6 antibody - ChIP Grade (ab53061) at 1/500 dilution (in TBS fro 16 hours at 4°C )Lane 1 : HeLa cellsLane 2 : Cell lysate (nuclear) of mouse embryonic stem cells at 100000 cellsSecondaryHRP-conjugated Donkey anti rabbit IgG polyclonal at 1/20000 dilutiondeveloped using the ECL techniquePerformed under non-reducing conditions.
ChIP analysis using ab53061 binding E2F6 in Mouse ES cell nuclear lysate. Samples were fixed with formaldehyde for 10 mins and incubated with primary antibody for 16 hours at 4°C at an antibody concentration of 1/500. Protein binding was detected using real-time PCR at both positive and negative control regions.See Abreview
Product References
Regulation of microRNA-mediated gene silencing by microRNA precursors. - Regulation of microRNA-mediated gene silencing by microRNA precursors.
Roy-Chaudhuri B, Valdmanis PN, Zhang Y, Wang Q, Luo QJ, Kay MA. Nat Struct Mol Biol. 2014 Sep;21(9):825-32.
Dnmt3b recruitment through E2F6 transcriptional repressor mediates germ-line gene - Dnmt3b recruitment through E2F6 transcriptional repressor mediates germ-line gene
Velasco G, Hube F, Rollin J, Neuillet D, Philippe C, Bouzinba-Segard H, Galvani A, Viegas-Pequignot E, Francastel C. Proc Natl Acad Sci U S A. 2010 May 18;107(20):9281-6. doi: