Anti-E3 SUMO-protein ligase PIAS4 antibody
| Name | Anti-E3 SUMO-protein ligase PIAS4 antibody |
|---|---|
| Supplier | Abcam |
| Catalog | ab58416 |
| Prices | $370.00 |
| Sizes | 100 µg |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Applications | IHC-P WB ELISA |
| Species Reactivities | Human, Mouse |
| Antigen | Synthetic peptide derived from the C-terminus of human PAIAS gamma |
| Description | Rabbit Polyclonal |
| Gene | PIAS4 |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
All lanes : Anti-E3 SUMO-protein ligase PIAS4 antibody (ab58416) at 1/500 dilutionLane 1 : Jurkat cell extract without immunizing peptideLane 2 : Jurkat cell extract with immunizing peptide
IHC image of ab58416 staining in human testis formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab58416, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
Product References
Caveolin-3 undergoes SUMOylation by the SUMO E3 ligase PIASy: sumoylation affects - Caveolin-3 undergoes SUMOylation by the SUMO E3 ligase PIASy: sumoylation affects
Fuhs SR, Insel PA. J Biol Chem. 2011 Apr 29;286(17):14830-41.
PKCzeta mediates disturbed flow-induced endothelial apoptosis via p53 - PKCzeta mediates disturbed flow-induced endothelial apoptosis via p53
Heo KS, Lee H, Nigro P, Thomas T, Le NT, Chang E, McClain C, Reinhart-King CA, King MR, Berk BC, Fujiwara K, Woo CH, Abe J. J Cell Biol. 2011 May 30;193(5):867-84.