Anti-EAAT1 antibody
| Name | Anti-EAAT1 antibody |
|---|---|
| Supplier | Abcam |
| Catalog | ab41751 |
| Prices | $390.00 |
| Sizes | 100 µg |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Applications | WB IHC-P ICC/IF ICC/IF |
| Species Reactivities | Mouse, Human, Rat, Bovine |
| Antigen | Synthetic peptide conjugated to KLH derived from within residues 1 - 100 of Human EAAT1 |
| Description | Rabbit Polyclonal |
| Gene | SLC1A3 |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
Anti-EAAT1 antibody (ab41751) at 1 µg/ml + Brain (Human) Tissue Lysate - adult normal tissue (ab29466) at 10 µgSecondaryIRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/10000 dilutionPerformed under reducing conditions.
ICC/IF image of ab41751 stained PC12 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab41751, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM. This antibody also gave a positive result in 4% PFA fixed (10 min) PC12 cells at 5µg/ml.
Immunohistochemical analysis of frozen mouse dorsal cortex tissue. EAAT1 (red) was stained with ab41751 at 1/500 dilution.
Product References
Impairment of neuronal glutamate uptake and modulation of the glutamate - Impairment of neuronal glutamate uptake and modulation of the glutamate
Russo R, Cavaliere F, Varano GP, Milanese M, Adornetto A, Nucci C, Bonanno G, Morrone LA, Corasaniti MT, Bagetta G. PLoS One. 2013 Aug 6;8(8):e69250.
Polydendrocytes display large lineage plasticity following focal cerebral - Polydendrocytes display large lineage plasticity following focal cerebral
Honsa P, Pivonkova H, Dzamba D, Filipova M, Anderova M. PLoS One. 2012;7(5):e36816.
In vitro evidence for the brain glutamate efflux hypothesis: brain endothelial - In vitro evidence for the brain glutamate efflux hypothesis: brain endothelial
Helms HC, Madelung R, Waagepetersen HS, Nielsen CU, Brodin B. Glia. 2012 May;60(6):882-93.