ab181036 staining EAAT1 in monkey brain tissue sections by Immunohistochemistry (PFA perfusion fixed frozen sections). Tissue samples were fixed by perfusion with paraformaldehyde, blocked with 10% serum at 24°C and antigen retrieval was by heat mediation in 10mM citrate acid + 0.05% Tween 20. The sample was incubated with primary antibody (1/100 in 2% donkey buffer) at 24°C for 13 hours. An Alexa Fluor® 647-conjugated donkey anti-rabbit polyclonal (1/100) was used as the secondary antibody.See Abreview
All lanes : Anti-EAAT1 antibody [EPR12686] (ab181036) at 1/1000 dilutionLane 1 : Mouse brain lysateLane 2 : Rat brain lysateLane 3 : Human cerebellum lysateLysates/proteins at 10 µg per lane.
Immunohistochemical staining of EAAT1 in paraffin-embedded human brain tissue using ab181036 at a 1/50 dilution.