Anti-EAAT2 antibody
| Name | Anti-EAAT2 antibody |
|---|---|
| Supplier | Abcam |
| Catalog | ab77039 |
| Prices | $385.00 |
| Sizes | 100 µg |
| Host | Mouse |
| Clonality | Monoclonal |
| Isotype | IgG2a |
| Applications | WB ELISA IHC-P |
| Species Reactivities | Human, Mouse, Rat, Cat, Dog, Orangutan |
| Antigen | Recombinant fragment: DEVSSLDAFL DLIRNLFPEN LVQACFQQIQ TVTKKVLVAP PPDEEANATS AVVSLLNETV TEVPEETKMV IKKGLEFKDG, corresponding to amino acids 160 - 239 of Human EAAT2 (NP_004162) with a 26 kDa tag Run BLAST with Run BLAST with |
| Description | Mouse Monoclonal |
| Gene | SLC1A2 |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
IHC image of EAAT2 staining in Human normal cerebral cortex formalin fixed paraffin embedded tissue section, performed on a Leica Bond™ system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab77039, 10µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
Anti-EAAT2 antibody (ab77039) at 1/500 dilution + recombinant tagged EAAT2 (the immunogen) at 0.2 µg/mlSecondarygoat anti-mouse IgG (H&L)-HRP conjugate at 1/5000 dilution
Anti-EAAT2 antibody (ab77039) at 1/500 dilution + HeLa cell lysate expressing EAAT2 at 25 µgSecondarygoat anti-mouse IgG (H&L)-HRP conjugate at 1/2500 dilution
Capture ELISA using ab77039 and recombinant tagged EAAT2