Flow cytometric analysis of 2% paraformaldehyde-fixed Jurkat cells labeling EBP1 with ab180603 at 1/20 dilution (red) compared to a Rabbit monoclonal IgG Isotype control (green), followed by Goat anti rabbit IgG (FITC) at 1/150 dilution.
All lanes : Anti-EBP1 antibody [EPR14568(B)] (ab180603) at 1/10000 dilutionLane 1 : 293T cell lysateLane 2 : HeLa cell lysateLane 3 : Jurkat cell lysateLane 4 : A431 cell lysateLysates/proteins at 20 µg per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution