Western blot analysis of Jurkat lysate immunoprecipitated with ab180602 at 1/30 dilution (Lane 1). Lane 2: Negative control. Anti-Rabbit IgG (HRP) secondary antibody, specific to the non-reduced form of IgG, used at 1/1500 dilution.
Flow cytometric analysis of 2% paraformaldehyde-fixed Jurkat cells labeling EBP1 with ab180602 at 1/20 dilution (red) compared to a Rabbit monoclonal IgG Isotype control (green), followed by Goat anti rabbit IgG (FITC) at 1/150 dilution.
Immunofluorescent analysis of 4% paraformaldehyde-fixed HeLa cells labeling EBP1 with 180602 at 1/100 dilution, followed by Goat anti rabbit IgG (Alexa Fluor® 555) secondary antibody at 1/200 dilution (Left panel). Right panel: Dapi staining.
Immunohistochemical analysis of paraffin-embedded Human colon adenocarcinoma tissue labeling EBP1 with ab180602 at 1/100 dilution, followed by prediluted ImmunoHistoprobe HRP Polymer for Rabbit IgG. Counter stained with Hematoxylin.
All lanes : Anti-EBP1 antibody [EPR14569(B)] (ab180602) at 1/20000 dilutionLane 1 : 293T lysateLane 2 : HeLa lysateLane 3 : Jurkat lysateLane 4 : A431 lysateLysates/proteins at 20 µg per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution