Anti-EBP50 antibody [EBP-10]
| Name | Anti-EBP50 antibody [EBP-10] |
|---|---|
| Supplier | Abcam |
| Catalog | ab9526 |
| Prices | $384.00 |
| Sizes | 100 µg |
| Host | Mouse |
| Clonality | Monoclonal |
| Isotype | IgG2b |
| Clone | EBP-10 |
| Applications | FC IP WB |
| Species Reactivities | Mouse, Human |
| Antigen | Recombinant full length protein (Human) |
| Description | Mouse Monoclonal |
| Gene | SLC9A3R1 |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
![All lanes : Anti-EBP50 antibody [EBP-10] (ab9526) at 2 µg/mlLane 1 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate Lane 2 : HeLa (Human epithelial carcinoma cell line) Whole Cell LysateLane 3 : Caco 2 (Human colonic carcinoma cell line) Whole Cell Lysate Lysates/proteins at 10 µg per lane.SecondaryGoat polyclonal to Mouse IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilutionObserved band size : 48 kDa (why is the actual band size different from the predicted?)](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_2/11439_EBP50-Primary-antibodies-ab9526-2.jpg)
All lanes : Anti-EBP50 antibody [EBP-10] (ab9526) at 2 µg/mlLane 1 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate Lane 2 : HeLa (Human epithelial carcinoma cell line) Whole Cell LysateLane 3 : Caco 2 (Human colonic carcinoma cell line) Whole Cell Lysate Lysates/proteins at 10 µg per lane.SecondaryGoat polyclonal to Mouse IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilutionObserved band size : 48 kDa (why is the actual band size different from the predicted?)
![Overlay histogram showing HepG2 cells stained with ab9526 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab9526, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG2b [PLPV219] (ab91366, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_2/11440_EBP50-Primary-antibodies-ab9526-3.jpg)
Overlay histogram showing HepG2 cells stained with ab9526 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab9526, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG2b [PLPV219] (ab91366, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.
Product References
Ezrin-anchored protein kinase A coordinates phosphorylation-dependent disassembly - Ezrin-anchored protein kinase A coordinates phosphorylation-dependent disassembly
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A putative RUNX1 binding site variant between SLC9A3R1 and NAT9 is associated - A putative RUNX1 binding site variant between SLC9A3R1 and NAT9 is associated
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Interaction between two adapter proteins, PAG and EBP50: a possible link between - Interaction between two adapter proteins, PAG and EBP50: a possible link between
Brdickova N, Brdicka T, Andera L, Spicka J, Angelisova P, Milgram SL, Horejsi V. FEBS Lett. 2001 Oct 26;507(2):133-6.