SLC9A3R1

Gene Symbol SLC9A3R1
Entrez Gene 9368
Alt Symbol EBP50, NHERF, NHERF-1, NHERF1, NPHLOP2
Species Human
Gene Type protein-coding
Description solute carrier family 9, subfamily A (NHE3, cation proton antiporter 3), member 3 regulator 1
Other Description Na(+)/H(+) exchange regulatory cofactor NHE-RF1|Na+/H+ exchange regulatory co-factor|ezrin-radixin-moesin binding phosphoprotein-50|ezrin-radixin-moesin-binding phosphoprotein 50|regulatory cofactor of Na(+)/H(+) exchanger|solute carrier family 9 (sodium/hydrogen exchanger), isoform 3 regulatory factor 1|solute carrier family 9 isoform A3 regulatory factor 1
Swissprots Q86WQ5 O43552 B3KY21 O14745
Accessions EAW89189 EAW89190 EAW89191 O14745 AF015926 AAC52084 AF036241 AAC04572 AI203594 AK026581 AK094467 BAG52871 AK128474 BAG54683 AK312498 BAG35400 BC001443 AAH01443 BC003361 AAH03361 BC011777 AAH11777 BC049220 AAH49220 BC053350 AAH53350 BP347679 BX648303 CN286501 CX761192 NM_004252 NP_004243
Function Scaffold protein that connects plasma membrane proteins with members of the ezrin/moesin/radixin family and thereby helps to link them to the actin cytoskeleton and to regulate their surface expression. Necessary for recycling of internalized ADRB2. Was first known to play a role in the regulation of the activity and subcellular location of SLC9A3. Necessary for cAMP-mediated phosphorylation and inhibition of SLC9A3. May enhance Wnt signaling. May participate in HTR4 targeting to microvilli (By similarity). Involved in the regulation of phosphate reabsorption in the renal proximal tubules. Involved in sperm capacitation. May participate in the regulation of the chloride and bicarbonate homeostasis in spermatozoa. {ECO:0000250, ECO:0000269|PubMed:10499588, ECO:0000269|PubMed:18784102, ECO:0000269|PubMed:9096337, ECO:0000269|PubMed:9430655}.
Subcellular Location Cytoplasm {ECO:0000250}. Apical cell membrane {ECO:0000250}. Endomembrane system; Peripheral membrane protein. Cell projection, filopodium. Cell projection, ruffle. Cell projection, microvillus. Note=Translocates from the cytoplasm to the apical cell membrane in a PODXL-dependent manner. Colocalizes with CFTR at the midpiece of sperm tail (By similarity). Colocalizes with actin in microvilli-rich apical regions of the syncytiotrophoblast. Found in microvilli, ruffling membrane and filopodia of HeLa cells. Present in lipid rafts of T- cells. {ECO:0000250}.
Tissue Specificity Detected in liver, kidney, pancreas, prostate, spleen, small intestine and placenta, in particular in the syncytiotrophoblast. {ECO:0000269|PubMed:9096337, ECO:0000269|PubMed:9314537}.

NHERF1 (A140) Antibody - 3382 from Cell Signaling Technology

Prices $246.00
Sizes 100 µl (10 western blots)
Host Rabbit
Clonality Polyclonal
Western blot analysis of extracts from MCF-7 and T-47D cells using NHERF1 (A140) Antibody.

NHERF1 (A310) Antibody - 3394 from Cell Signaling Technology

Prices $246.00
Sizes 100 µl (10 western blots)
Host Rabbit
Clonality Polyclonal
Western blot analysis of extracts from MCF-7 and T-47D cells using NHERF1 (A310) Antibody.

NHERF1 (D1C10) Rabbit mAb - 8601 from Cell Signaling Technology

Prices $246.00
Sizes 100 µl (10 western blots)
Host Rabbit
Clonality Monoclonal
Clone D1C10
Western blot analysis of extracts from T-47D and MCF7 cells using NHERF1 (D1C10) Rabbit mAb.

NHERF-1 (A-7) - sc-271552 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
NHERF-1 (A-7): sc-271552. Immunoperoxidase staining of formalin fixed, paraffin-embedded human gall bladder tissue showing cytoplasmic staining of glandular cells.

NHERF-1 (EBP-10) - sc-51684 from Santa Cruz Biotechnology

Prices $279.00
Sizes 100 µg/ml
Host Mouse
Clonality Monoclonal
NHERF-1 (EBP-10): sc-51684. Western blot analysis of NHERF-1 expression in non-transfected: sc-110760 (A) and human NHERF-1 transfected: sc-110574 (B) 293 whole cell lysates.

NHERF-1 (H-100) - sc-134485 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Rabbit
Clonality Polyclonal
NHERF-1 (H-100): sc-134485. Immunoperoxidase staining of formalin fixed, paraffin-embedded human kidney tissue showing cytoplasmic staining of cells in glomeruli and tubules.

NHERF-1 (6D364) - sc-71698 from Santa Cruz Biotechnology

Prices $279.00
Sizes 100 µg/ml
Host Mouse
Clonality Monoclonal
NHERF-1 (6D364): sc-71698. Western blot analysis of NHERF-1 expression in non-transfected: sc-110760 (A) and human NHERF-1 transfected: sc-110574 (B) 293 whole cell lysates.

NHERF-1 (4i337) - sc-71697 from Santa Cruz Biotechnology

Prices $279.00
Sizes 100 µg/ml
Host Mouse
Clonality Monoclonal
NHERF-1 (4i337): sc-71697. Western blot analysis of NHERF-1 expression in non-transfected: sc-110760 (A) and human NHERF-1 transfected: sc-110574 (B) 293 whole cell lysates.

NHERF-1 (6) - sc-136228 from Santa Cruz Biotechnology

Prices $279.00
Sizes 50 µg/0.5 ml
Host Mouse
Clonality Monoclonal
NHERF-1 (6): sc-136228. Western blot analysis of NHERF-1 expression in human endothelial whole cell lysate.

Anti-EBP50 antibody [EBP-10] - ab9526 from Abcam

Prices $384.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal
Clone EBP-10
All lanes : Anti-EBP50 antibody [EBP-10] (ab9526) at 2 µg/mlLane 1 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate Lane 2 : HeLa (Human epithelial carcinoma cell line) Whole Cell LysateLane 3 : Caco 2 (Human colonic carcinoma cell line) Whole Cell Lysate Lysates/proteins at 10 µg per lane.SecondaryGoat polyclonal to Mouse IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilutionObserved band size : 48 kDa (why is the actual band size different from the predicted?)

Anti-EBP50 antibody [EPR5562] - ab109430 from Abcam

Prices $379.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EPR5562
All lanes : Anti-EBP50 antibody [EPR5562] (ab109430) at 1/1000 dilutionLane 1 : HepG2 cell lysateLane 2 : 293T cell lysateLane 3 : Jurkat cell lysateLane 4 : C6 cell lysateLane 5 : PC12 cell lysateLane 6 : MCF7 cell lysateLysates/proteins at 10 µg per lane.

Anti-EBP50 antibody [EPR5563] - ab133599 from Abcam

Prices $387.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EPR5563
Overlay histogram showing HepG2 cells stained with ab133599 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab133599, 1/1000 dilution) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-rabbit IgG (H&L) (ab150077) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (0.1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. This antibody gave a positive signal in HepG2 cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.

Anti-EBP50 antibody - BSA and Azide free - ab59161 from Abcam

Prices $376.00
Sizes 50 µg
Host Chicken
Clonality Polyclonal
Anti-EBP50 antibody - BSA and Azide free (ab59161) at 1/500 dilution + placenta protein extract at 1 µgSecondaryGoat anti-IgY-HRP at 1/1000 dilution

Anti-SLC9A3R1 / NHERF1 / EBP50 Antibody (Biotin) - LS-C36219 from LifeSpan Bioscience

Prices $485.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal

Anti-SLC9A3R1 / NHERF1 / EBP50 Antibody - LS-C15005 from LifeSpan Bioscience

Prices $435.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal

Anti-SLC9A3R1 / NHERF1 / EBP50 Antibody - LS-C44851 from LifeSpan Bioscience

Prices $305.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal

Anti-SLC9A3R1 / NHERF1 / EBP50 Antibody (C-Terminus) - LS-C37496 from LifeSpan Bioscience

Prices $415.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal

Anti-SLC9A3R1 / NHERF1 / EBP50 Antibody (C-Terminus) - LS-C46891 from LifeSpan Bioscience

Prices $315.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal

Anti-SLC9A3R1 / NHERF1 / EBP50 Antibody - LS-C128694 from LifeSpan Bioscience

Prices $415.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal

Anti-SLC9A3R1 / NHERF1 / EBP50 Antibody (Ala140) - LS-C73288 from LifeSpan Bioscience

Prices $405.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal

Anti-SLC9A3R1 / NHERF1 / EBP50 Antibody - LS-C128695 from LifeSpan Bioscience

Prices $405.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal

Anti-SLC9A3R1 / NHERF1 / EBP50 Antibody - LS-C153193 from LifeSpan Bioscience

Prices $410.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal

Anti-SLC9A3R1 / NHERF1 / EBP50 Antibody - LS-C183451 from LifeSpan Bioscience

Prices $435.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal

Anti-SLC9A3R1 / NHERF1 / EBP50 Antibody (clone EBP-10) - LS-C202427 from LifeSpan Bioscience

Prices $335.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal
Clone EBP-10

Anti-SLC9A3R1 / NHERF1 / EBP50 Antibody (aa175-205, AP) - LS-C269582 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal
Search more