Anti-EBP50 antibody [EPR5563]

• Supplier: Abcam
• Catalog: ab133599
• Prices: $387.00
• Sizes: 100 µl
• Host: Rabbit
• Clonality: Monoclonal
• Isotype: IgG
• Clone: EPR5563
• Applications: WB IHC-P ICC/IF IP FC
• Species Reactivities: Mouse, Rat, Human
• Antigen: Synthetic peptide corresponding to a region within the C terminus of Human EBP50
• Blocking Peptide: EBP50 peptide
• Description: Rabbit Monoclonal
• Gene: SLC9A3R1
• Conjugate: Unconjugated

Product images

Overlay histogram showing HepG2 cells stained with ab133599 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab133599, 1/1000 dilution) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-rabbit IgG (H&L) (ab150077) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (0.1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. This antibody gave a positive signal in HepG2 cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.

All lanes : Anti-EBP50 antibody [EPR5563] (ab133599) at 1/10000 dilutionLane 1 : HepG2 cell lysateLane 2 : MCF7 cell lysateLysates/proteins at 20 µg per lane.SecondaryHRP labelled goat anti-rabbit at 1/2000 dilution

Immunohistochemical analysis of paraffin-embedded Human kidney tissue labelling EBP50 with ab133599 at 1/50.

Immunohistochemical analysis of paraffin-embedded Human placenta tissue labelling EBP50 with ab133599 at 1/50.

Immunocytochemical analysis of HepG2 cells labelling EBP50 with ab133599 at 1/100.

Equilibrium disassociation constant (KD)Learn more about KD Click here to learn more about KD