Anti-EDC3 antibody
| Name | Anti-EDC3 antibody |
|---|---|
| Supplier | Abcam |
| Catalog | ab57780 |
| Prices | $370.00 |
| Sizes | 100 µg |
| Host | Mouse |
| Clonality | Monoclonal |
| Isotype | IgG1 |
| Applications | WB FC |
| Species Reactivities | Human |
| Antigen | Recombinant full length protein, corresponding to amino acids 1-509 of Human EDC3 |
| Description | Mouse Monoclonal |
| Gene | EDC3 |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
![Overlay histogram showing HeLa cells stained with ab57780 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab57780, 0.1μg/1x106 cells) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-mouse IgG (H&L) (ab150113) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_2/11576_ab57780-2-ab57780FC.jpg)
Overlay histogram showing HeLa cells stained with ab57780 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab57780, 0.1μg/1x106 cells) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-mouse IgG (H&L) (ab150113) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.
EDC3 antibody (ab57780) at 1ug/lane + HeLa cell lysate at 25ug/lane.
Product References
Human DDX6 effects miRNA-mediated gene silencing via direct binding to CNOT1. - Human DDX6 effects miRNA-mediated gene silencing via direct binding to CNOT1.
Rouya C, Siddiqui N, Morita M, Duchaine TF, Fabian MR, Sonenberg N. RNA. 2014 Sep;20(9):1398-409.
Phosphorylation of mRNA decapping protein Dcp1a by the ERK signaling pathway - Phosphorylation of mRNA decapping protein Dcp1a by the ERK signaling pathway
Chiang PY, Shen YF, Su YL, Kao CH, Lin NY, Hsu PH, Tsai MD, Wang SC, Chang GD, Lee SC, Chang CJ. PLoS One. 2013 Apr 18;8(4):e61697.
Human Pat1b connects deadenylation with mRNA decapping and controls the assembly - Human Pat1b connects deadenylation with mRNA decapping and controls the assembly
Ozgur S, Chekulaeva M, Stoecklin G. Mol Cell Biol. 2010 Sep;30(17):4308-23.