Immunocytochemistry/Immunofluorescence analysis of U2OS cells labelling EDD with ab175810 at 1:1000. Cells were fixed with PFA and permeabilized with methanol for 2 minutes. Cells were incubated with the primary anitbody overnight at room temperature. Top - EDD, Middle - DAPI, Bottom - Merge.
Immunohistochemistry (formalin/PFA-fixed paraffin-embedded) analysis of Human linfoid tissue labelling EDD with ab175810 at 1:1000. Antigen retrieval was by microwave boiling for 7 minutes in 10mM TrisHCl, pH9. Top - 4X, Middle - 10X, Bottom - 20X.
Immunoprecipitation analysis of U2OS cells labelling EED with ab175810. Protein A beads were crosslinked to the serum.
All lanes : Anti-EDD antibody (ab175810)Lane 1 : Control siLane 2 : UBR5 siLane 3 : NIH 3T3 cell lysate at 40 µgLane 4 : PC12 cell lysate at 40 µg