Anti-EDG1 antibody
| Name | Anti-EDG1 antibody |
|---|---|
| Supplier | Abcam |
| Catalog | ab23695 |
| Prices | $390.00 |
| Sizes | 250 µg |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Applications | IP WB IHC-P ICC/IF |
| Species Reactivities | Mouse, Rat, Human, Pig |
| Antigen | Synthetic peptide: ISKASRSSEKSLA , corresponding to amino acids 241-253 of Human EDG1 Run BLAST with Run BLAST with |
| Description | Rabbit Polyclonal |
| Gene | S1PR1 |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
All lanes : Anti-EDG1 antibody (ab23695) at 2 µg/mlLane 1 : 30ug of human liver microsomes.Lane 2 : 30ug mouse brain homogenate.Lane 3 : 30ug pig liver 10000 x g supernatantLane 4 : 30ug rat brain homogenate.
Immunohistochemistry performed on formalin fixed, paraffin embedded sections of neuro2-A cells with ab23695 (8ug/ml). The secondary antibody was a cy3 conjugated donkey anti rabbit antibody.
![EDG1 was immunoprecipitated using 0.5mg Mouse Brain tissue lysate, 5µg of Rabbit polyclonal to EDG1 and 50µl of protein G magnetic beads (+). No antibody was added to the control (-). The antibody was incubated under agitation with Protein G beads for 10min, Mouse Brain tissue lysate lysate diluted in RIPA buffer was added to each sample and incubated for a further 10min under agitation.Proteins were eluted by addition of 40µl SDS loading buffer and incubated for 10min at 70oC; 10µl of each sample was separated on a SDS PAGE gel, transferred to a nitrocellulose membrane, blocked with 5% BSA and probed with ab23695.Secondary: Mouse monoclonal [SB62a] Secondary Antibody to Rabbit IgG light chain (HRP) (ab99697).Band: 51kDa; EDG1](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_2/11637_EDG1-Primary-antibodies-ab23695-6.jpg)
EDG1 was immunoprecipitated using 0.5mg Mouse Brain tissue lysate, 5µg of Rabbit polyclonal to EDG1 and 50µl of protein G magnetic beads (+). No antibody was added to the control (-). The antibody was incubated under agitation with Protein G beads for 10min, Mouse Brain tissue lysate lysate diluted in RIPA buffer was added to each sample and incubated for a further 10min under agitation.Proteins were eluted by addition of 40µl SDS loading buffer and incubated for 10min at 70oC; 10µl of each sample was separated on a SDS PAGE gel, transferred to a nitrocellulose membrane, blocked with 5% BSA and probed with ab23695.Secondary: Mouse monoclonal [SB62a] Secondary Antibody to Rabbit IgG light chain (HRP) (ab99697).Band: 51kDa; EDG1
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