Anti-EEA1 antibody [EPR4245] - Early Endosome Marker (HRP)

• Supplier: Abcam
• Catalog: ab195553
• Prices: $425.00
• Sizes: 100 µl
• Host: Rabbit
• Clonality: Monoclonal
• Isotype: IgG
• Clone: EPR4245
• Applications: IHC-P WB
• Species Reactivities: Mouse, Human, Rat
• Antigen: Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) corresponding to Human EEA1 (N terminal)
• Description: Rabbit Monoclonal
• Gene: EEA1
• Conjugate: HRP

Product images

All lanes : Anti-EEA1 antibody [EPR4245] - Early Endosome Marker (HRP) (ab195553) at 1/5000 dilutionLane 1 : NIH 3T3 (Mouse embryonic fibroblast cell line) Whole Cell LysateLane 2 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate (ab150035)Lysates/proteins at 10 µg per lane.developed using the ECL techniquePerformed under reducing conditions.

IHC image of EEA1 staining in a section of formalin-fixed paraffin-embedded normal human colon tissue*. The section was pre-treated using pressure cooker heat mediated antigen retrieval with sodium citrate buffer (pH6) for 30mins, and incubated overnight at +4°C with ab195553 at 1/500 dilution. DAB was used as the chromogen (ab103723), diluted 1/100 and incubated for 10min at room temperature. The section was counterstained with haematoxylin and mounted with DPX. The inset negative control image is taken from an identical assay without primary antibody.For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.*Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre